Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, Lodi, 26900, Italy.
Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università 6, Lodi, 26900, Italy.
Res Vet Sci. 2024 Mar;169:105164. doi: 10.1016/j.rvsc.2024.105164. Epub 2024 Feb 1.
Mediterranean area represents the main habitat of Testudo hermanni. Clinical signs of disease of these tortoises are non-specific, making the hematology results crucial in revealing underlying pathological conditions. However, accurate automated identification of blood cell populations is hampered by the presence of nucleated erythrocytes (NRBC) and thrombocytes (Thr), necessitating manual methods such as counting chambers. The aim of the study was to assess the performance of the novel automated hematology analyzer Sysmex XN-1000 V, which includes a a specific channel (WNR) for counting NRBC, in accurately identify and quantify the different blood cell populations of Testudo hermanni. Additionally, its agreement with manual counts was evaluated. Fifty heparinized blood samples were initially counted using the Neubauer improved chamber and then analysed twice with Sysmex XN-1000 V. Thirteen out of 50 samples were instrumentally counted again after 48 h to assess the inter-assay precision. All WNR scattergrams were re-analysed using an ad hoc gate panel to differentiate two populations: NRBCs (weak fluorescence signal) and WBC + Thr (high fluorescence signal). Sysmex XN-1000 V demonstrated optimal intra- and inter-assay precision for NRBCs (CV 0.98% ± 1.96; 1.31% ± 2.98) and moderate precision for WBC + Thr (CV 9.24% ± 16.61; 12.69% ± 10.35). No proportional nor constant errors were observed between the methods for both the populations. The instrumental NRBC counts were consistently slightly lower, while WBC + Thr counts were slightly higher compared to manual counts. These findings suggest that Sysmex XN-1000 V can be used for analyzing cell populations in heparinized blood of Testudo hermanni. However, specific instrumental reference intervals are suggested.
地中海地区是赫曼陆龟的主要栖息地。这些陆龟的疾病临床症状是非特异性的，因此血液学结果对于揭示潜在的病理状况至关重要。然而，由于存在有核红细胞（NRBC）和血小板（Thr），准确地自动识别血细胞群体受到了阻碍，需要使用手动方法，例如计数室。本研究的目的是评估新型自动血液分析仪 Sysmex XN-1000 V 的性能，该仪器包括一个专门用于计数 NRBC 的通道（WNR），以准确识别和定量赫曼陆龟的不同血细胞群体。此外，还评估了其与手动计数的一致性。最初使用改良的牛鲍氏计数板对 50 份肝素化血液样本进行计数，然后使用 Sysmex XN-1000 V 分析两次。将 50 个样本中的 13 个在 48 小时后再次进行仪器计数，以评估批内精密度。使用专门的门控面板对所有 WNR 散点图进行重新分析，以区分两个群体：NRBC（弱荧光信号）和 WBC+Thr（高荧光信号）。XN-1000 V 对 NRBC（CV 0.98%±1.96；1.31%±2.98）的内和批间精密度最佳，对 WBC+Thr（CV 9.24%±16.61；12.69%±10.35）的精密度为中等。两种方法之间均未观察到比例或恒定误差。与手动计数相比，仪器计数的 NRBC 数量始终略低，而 WBC+Thr 计数略高。这些发现表明，XN-1000 V 可用于分析赫曼陆龟肝素化血液中的细胞群体。然而，建议建立特定的仪器参考区间。
