Ekejindu G O, Ekundare F O, Magaji Y
Vet Immunol Immunopathol. 1985 Dec;10(4):333-9. doi: 10.1016/0165-2427(85)90022-4.
A direct haemagglutination assay for antibodies to sheep red blood cells (SRBC) was used to assess the response of rats infected with Trypanosoma brucei gambiense. Whereas uninfected rats showed an efficient primary and secondary immune response to SRBC, trypanosome-infected rats displayed depressed antibody response starting about six days after infection. Infected rats failed to respond to a challenge dose of SRBC given 14 days after infection while uninfected control animals responded with an increased level of antibody production. These observations showed that T. b. gambiense infection inhibited both primary and secondary immune response to SRBC in rats. The result of this experiment is very important with regard to serological methods used to detect increasing levels of antibody production for diagnosis of diseases caused by bacterial and viral pathogens. In a concurrent trypanosome infection such increasing antibody levels would not be observed, leading to inaccurate diagnosis. Thus trypanosomiasis infection should be excluded under field conditions before the value of a serological diagnosis can be fully utilized.
采用针对绵羊红细胞（SRBC）抗体的直接血凝试验，评估感染布氏冈比亚锥虫大鼠的反应。未感染的大鼠对SRBC表现出有效的初次和二次免疫反应，而感染锥虫的大鼠在感染约六天后开始出现抗体反应降低。感染的大鼠在感染后14天给予SRBC激发剂量时无反应，而未感染的对照动物抗体产生水平升高。这些观察结果表明，布氏冈比亚锥虫感染抑制了大鼠对SRBC的初次和二次免疫反应。该实验结果对于用于检测抗体产生水平升高以诊断细菌和病毒病原体引起疾病的血清学方法非常重要。在同时存在锥虫感染的情况下，不会观察到这种抗体水平升高，从而导致诊断不准确。因此，在充分利用血清学诊断价值之前，应在野外条件下排除锥虫病感染。
