College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China.
Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, China.
ACS Appl Mater Interfaces. 2024 Feb 21;16(7):8378-8390. doi: 10.1021/acsami.3c16917. Epub 2024 Feb 7.
Slow healing at the tendon-bone interface is a prominent factor in the failure of tendon repair surgeries. The development of functional biomaterials with 3D gradient structures is urgently needed to improve tendon-bone integration. The crystalline form of hydroxyapatite (HAP) has a crucial impact on cell behavior, which directly influences protein adsorption, such as bone morphogenetic protein 2, the adhesion, proliferation, and osteogenic differentiation with cells. This work aimed to generate gradient mineral structures in situ by stabilizing calcium and phosphate ions using a polymer-induced liquid precursor process. To regulate the crystalline growth of HAP at the interface of β-chitin, this work made use of the surface properties of the organic matrix found in cuttlefish bone. These techniques allowed us to prepare an organic-inorganic composite gradient scaffold comprising plate-like HAP mineralized in situ on the surface of the scaffold and fibrous HAP in the scaffold's interior. Organic-inorganic composite gradient materials are anticipated for use in tendon-bone healing produced via the in situ construction of gradient-distributed HAP mineralization layers having varying crystalline morphologies on chitin scaffolds that possess a three-dimensional bionic structure.
在肌腱-骨界面处的愈合缓慢是肌腱修复手术失败的一个突出因素。急需开发具有 3D 梯度结构的功能性生物材料，以改善肌腱-骨整合。羟基磷灰石（HAP）的晶体形式对细胞行为有至关重要的影响，这直接影响蛋白质的吸附，如骨形态发生蛋白 2，与细胞的黏附、增殖和成骨分化。本工作旨在通过使用聚合物诱导的液体前体工艺稳定钙和磷酸盐离子，在原位产生梯度矿化结构。为了调节 HAP 在 β-几丁质界面处的晶体生长，本工作利用了鱿鱼骨中有机基质的表面性质。这些技术使我们能够制备一种有机-无机复合梯度支架，该支架在支架表面原位矿化有板状 HAP，在支架内部有纤维状 HAP。有机-无机复合梯度材料有望用于通过在具有三维仿生结构的几丁质支架上原位构建具有不同晶体形态的梯度分布 HAP 矿化层来促进肌腱-骨愈合。
