Giannetto Claudia, Arfuso Francesca, Rizzo Maria, Giudice Elisabetta, Calapai Fabrizio, Guercio Annalisa, Macaluso Giusi, Giacchino Ilenia, Piccione Giuseppe, Cannella Vincenza
Department of Veterinary Sciences, University of Messina, Messina, Italy.
Department of Chemical, Pharmaceutical and Environmental Scieces, University of Messina, Messina, Italy.
Chronobiol Int. 2024 Mar;41(3):369-377. doi: 10.1080/07420528.2024.2315217. Epub 2024 Feb 7.
Dogs are the common pets adopted by humans, and their circadian behavior and physiology are influenced by human habits. In many families, there is a change of lifestyle with respect to the natural daylight (NDL) cycle. Exposure to constant light disrupts some central and peripheral circadian rhythms. The aim of the present study was to improve the knowledge about the circadian changes of clock components in the peripheral blood in dogs housed under NDL and constant light (LL) conditions. Blood samples were collected on five female Beagle dogs (2 years old, 14 ± 0.5 kg) every 4 hours for a 24-hour period during an NDL (Sunrise 05:05 h - Sunset 20:55 h) and 24-hour period of constant light (LL). Blood samples were stored in a PAX gene Blood RNA Tube, real-time RT-quantitative polymerase chain reaction was performed to determine gene expression. During the NDL, all genes investigated showed robust diurnal daily rhythmicity. During the constant light, only maintained its daily rhythmicity. acrophase was observed close to sunrise (ZT 0) and was statistically different from the other clock genes except for . daily oscillations were not statistically significant. No differences were observed among the clock genes tested in the amplitude and robustness values. Our results can be considered preliminary data to provide new insights into the adaptation mechanism of the canine peripheral circadian clock. The persistence of gene expression during the LL indicated the presence of an endogenously generated signal in blood. Because peripheral blood is an easily accessible sample in dogs, the analysis of clock gene expression in this tissue could be useful to investigate the adaptive capacity of this species housed in different environmental conditions linked to the owner's lifestyle.
狗是人类常见的宠物，它们的昼夜行为和生理机能受人类习惯影响。在许多家庭中，相对于自然日光（NDL）周期，生活方式发生了变化。持续光照会扰乱一些中枢和外周昼夜节律。本研究的目的是增进对处于自然日光和持续光照（LL）条件下的犬外周血中生物钟成分昼夜变化的了解。在自然日光周期（日出05:05 - 日落20:55）和持续光照的24小时期间，每4小时采集5只雌性比格犬（2岁，14±0.5千克）的血样。血样储存在PAX基因血液RNA管中，进行实时RT定量聚合酶链反应以测定基因表达。在自然日光周期期间，所有研究的基因均显示出强烈的昼夜节律性。在持续光照期间，只有 维持其昼夜节律性。 观察到的峰相位接近日出（ZT 0），并且除 外，与其他生物钟基因在统计学上有差异。 的每日振荡在统计学上不显著。在所测试的生物钟基因的振幅和稳健性值之间未观察到差异。我们的结果可被视为初步数据，为犬外周生物钟的适应机制提供新的见解。在持续光照期间 基因表达的持续存在表明血液中存在内源性产生的信号。由于外周血是犬类易于获取的样本，分析该组织中的生物钟基因表达可能有助于研究该物种在与主人生活方式相关的不同环境条件下的适应能力。
