State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, P. R. China.
Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, P. R. China.
J Agric Food Chem. 2024 Feb 21;72(7):3793-3799. doi: 10.1021/acs.jafc.3c07535. Epub 2024 Feb 7.
Carotenoids, as a type of tetraterpene compound, have been widely used in food, medical, and health areas owing to their antioxidant, immune enhancement, and disease risk reduction effects. is a promising oleaginous red yeast that can industrially synthesize carotenoids. In this study, the effects of different light exposure times and intervals on carotenoid production by Z11 were first investigated. Results showed that a higher carotenoid content (1.29 mg/g) can be achieved when Z11 was exposed to light for 12 h per day, which was increased by 1.98 times compared with that of dark cultivation. Transcriptome profiling revealed that light stress could effectively promote the gene expression levels of and in the carotenoid biosynthesis pathway and in the DNA photolysis pathway of . This work will provide a molecular foundation to further improve the production efficiency of carotenoids by genetic engineering.
类胡萝卜素作为一种四萜化合物，由于其具有抗氧化、增强免疫力和降低疾病风险的作用，已被广泛应用于食品、医疗和保健领域。 是一种有前途的产油红色酵母，可以工业合成类胡萝卜素。在这项研究中，首先研究了不同光照时间和间隔对 Z11 产类胡萝卜素的影响。结果表明，当 Z11 每天光照 12 小时时，可获得更高的类胡萝卜素含量（1.29mg/g），比黑暗培养时增加了 1.98 倍。转录组谱分析显示，光照胁迫能有效促进类胡萝卜素生物合成途径中 和 基因以及 基因的表达水平， 基因的 DNA 光解途径。这项工作将为通过基因工程进一步提高类胡萝卜素的生产效率提供分子基础。
