用甘蔗蜜发酵液培养粘红酵母突变株生产脂类和类胡萝卜素。
Lipid and carotenoid production by the Rhodosporidium toruloides mutant in cane molasses.
机构信息
The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou, China.
The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou, China.
出版信息
Bioresour Technol. 2021 Apr;326:124816. doi: 10.1016/j.biortech.2021.124816. Epub 2021 Feb 3.
Cane molasses is beneficial for lipid and carotenoid production in microalgae. We made a survey for the lipid and carotenoid production profile of R. toruloides M18 (MT) with various concentrations of molasses under nitrogen-deficited conditions. The production of α-linolenate and torularhodin from MT were 1.22- and 14.68-fold higher than those of the wild-type strain. We observed that molasses at concentrations of 35 g/L and 70 g/L represented a cheap and environmentally friendly strategy for producing lipids and carotenoids. Transcriptome and WGCNA analysis demonstrated that the genes relevant to the lipid and carotenoid production, including MYB, bHLH, Δ-4 desaturase, Δ-12 desaturase and FA2H, were significantly highly expressed. The results indicated that molasses could represent an inexpensive means for achieving high lipids and carotenoids production in R. toruloides.
甘蔗糖蜜有利于微藻中脂类和类胡萝卜素的生产。我们在氮饥饿条件下，用不同浓度的甘蔗糖蜜对 R. toruloides M18（MT）的脂类和类胡萝卜素的生产情况进行了调查。MT 产生的α-亚麻酸和 torularhodin 分别比野生型菌株高 1.22 倍和 14.68 倍。我们发现，35g/L 和 70g/L 的糖蜜浓度代表了一种廉价且环保的生产脂类和类胡萝卜素的策略。转录组和 WGCNA 分析表明，与脂类和类胡萝卜素生产相关的基因，包括 MYB、bHLH、Δ-4 去饱和酶、Δ-12 去饱和酶和 FA2H，表达水平显著升高。结果表明，甘蔗糖蜜可以作为一种廉价的手段，实现 R. toruloides 中脂类和类胡萝卜素的高产。
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