Zhou Xiaoyu, Wang Zhuofan, Li Shuai, Rong Xianle, Bu Jiexun, Liu Qiang, Ouyang Zheng
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China.
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Science. 2024 Feb 9;383(6683):612-618. doi: 10.1126/science.adj8342. Epub 2024 Feb 8.
Conventional mass spectrometry does not distinguish between enantiomers, or mirror-image isomers. Here we report a technique to break the chiral symmetry and to differentiate enantiomers by inducing directional rotation of chiral gas-phase ions. Dual alternating current excitations were applied to manipulate the motions of trapped ions, including the rotation around the center of mass and macro movement around the center of the trap. Differences in collision cross section were induced, which could be measured by ion cloud profiling at high resolutions above 10,000. High-field ion mobility and tandem mass spectrometry analyses of the enantiomers were combined and implemented by using a miniature ion trap mass spectrometer. The effectiveness of the developed method was demonstrated with a variety of organic compounds including amino acids, sugars, and several drug molecules, as well as a proof-of-principle ligand optimization study for asymmetric hydrogenation.
传统质谱法无法区分对映体或镜像异构体。在此，我们报告一种通过诱导手性气相离子的定向旋转来打破手性对称性并区分对映体的技术。施加双交流激发以操纵捕获离子的运动，包括围绕质心的旋转和围绕阱中心的宏观移动。诱导出碰撞截面的差异，这可以通过高于10000的高分辨率离子云分析来测量。通过使用微型离子阱质谱仪，将对映体的高场离子迁移率和串联质谱分析相结合并实施。所开发方法的有效性在包括氨基酸、糖类和几种药物分子在内的多种有机化合物上得到了证明，以及用于不对称氢化的原理验证配体优化研究。
