Synergistic enantioseparation system for racemate amlodipine based on a novel nanomaterial synthesized by chiral molecularly imprinted polymer and chiral metal-organic framework in capillary electrochromatography.

A capillary coating column was prepared for capillary electrochromatography (CEC) using a novel nanomaterial synthesized by chiral metal-organic frameworks (CMOFs) (Co(L-Trp)(INT)(HO)(ClO) and chiral molecularly imprinted polymers (CMIPs) as the stationary phase for enantioseparation of amlodipine. The rigid skeleton of CMOFs can be used as a support to reduce the deformation and collapse of CMIPs pores, the high specific surface area of CMOFs can increase the loading of molecular imprinting, while the viscosity of CMIPs improves the stability of stationary phases connected to the inner wall of capillaries. Compared with the capillary-coated columns with CMOFs and CMIPs as stationary phases alone, the novel capillary-coated columns utilizing the synergistic effect of  CMIP@CMOF stationary phases showed a greatly improved chiral recognition (resolution: 0/0 → 5.79). The new nanomaterials synthesized by CMOFs and CMIPs as the stationary phase of CEC chiral resolution system show broad application prospects and potentials in the field of chiral drug/compound separation.