Guo Chunyan, Miao Pandeng, Yan Yifan, Li Xiaoqi, Du Shuaijing, Du Yingxiang
Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009, PR China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, PR China.
Mikrochim Acta. 2025 Jun 28;192(7):463. doi: 10.1007/s00604-025-07242-6.
A capillary coating column was prepared for capillary electrochromatography (CEC) using a novel nanomaterial synthesized by chiral metal-organic frameworks (CMOFs) (Co(L-Trp)(INT)(HO)(ClO) and chiral molecularly imprinted polymers (CMIPs) as the stationary phase for enantioseparation of amlodipine. The rigid skeleton of CMOFs can be used as a support to reduce the deformation and collapse of CMIPs pores, the high specific surface area of CMOFs can increase the loading of molecular imprinting, while the viscosity of CMIPs improves the stability of stationary phases connected to the inner wall of capillaries. Compared with the capillary-coated columns with CMOFs and CMIPs as stationary phases alone, the novel capillary-coated columns utilizing the synergistic effect of CMIP@CMOF stationary phases showed a greatly improved chiral recognition (resolution: 0/0 → 5.79). The new nanomaterials synthesized by CMOFs and CMIPs as the stationary phase of CEC chiral resolution system show broad application prospects and potentials in the field of chiral drug/compound separation.
采用手性金属有机框架（CMOFs）（Co(L-Trp)(INT)(HO)(ClO)）合成的新型纳米材料和手性分子印迹聚合物（CMIPs）作为固定相，制备了一种用于毛细管电色谱（CEC）的毛细管涂层柱，用于氨氯地平对映体的分离。CMOFs的刚性骨架可作为支撑，减少CMIPs孔的变形和塌陷，CMOFs的高比表面积可增加分子印迹的负载量，而CMIPs的粘度提高了连接到毛细管内壁的固定相的稳定性。与单独以CMOFs和CMIPs为固定相的毛细管涂层柱相比，利用CMIP@CMOF固定相协同效应的新型毛细管涂层柱显示出极大提高的手性识别能力（分辨率：0/0→5.79）。由CMOFs和CMIPs合成的新型纳米材料作为CEC手性拆分系统的固定相，在手性药物/化合物分离领域显示出广阔的应用前景和潜力。
