Jagota S K, Dani H M
Aust J Exp Biol Med Sci. 1985 Dec;63 ( Pt 6):683-9. doi: 10.1038/icb.1985.71.
Rat liver microsomes can be prepared by the addition of glutathione to post-mitochondrial supernatant (PMS) and its subsequent sedimentation at 10,000 g. These microsomes seem to be unaffected with respect to their major components, and substantial loss of their ribosomes on treatments with various degranulating reagents shows that they contain intact ribosomes. The indices of ribosomal attachment show that these preparations contain more ribosomes per unit area of membrane surface as compared to those prepared at high g forces. High values of per cent degranulation by incubating these microsomes with coded carcinogens and failure of non-carcinogens to cause this effect show that the technique can be used efficiently for quick, inexpensive and more accurate prediction of carcinogens in human environments.
通过向线粒体后上清液（PMS）中添加谷胱甘肽并随后在10,000 g下进行沉降，可以制备大鼠肝脏微粒体。这些微粒体的主要成分似乎未受影响，并且在用各种脱粒试剂处理后其核糖体大量损失，这表明它们含有完整的核糖体。核糖体附着指数表明，与在高离心力下制备的微粒体相比，这些制剂每单位膜表面积含有更多的核糖体。通过将这些微粒体与编码致癌物一起孵育，脱粒百分比很高，而非致癌物未能产生这种效果，这表明该技术可有效地用于快速、廉价且更准确地预测人类环境中的致癌物。
