Laboratory of Developmental Biology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Kagoshima, Japan.
Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.1038/s41598-024-53687-9.
Mus minutoides is one of the smallest mammals worldwide; however, the regulatory mechanisms underlying its dwarfism have not been examined. Therefore, we aimed to establish M. minutoides induced pluripotent stem cells (iPSCs) using the PiggyBac transposon system for applications in developmental engineering. The established M. minutoides iPSCs were found to express pluripotency markers and could differentiate into neurons. Based on in vitro differentiation analysis, M. minutoides iPSCs formed embryoid bodies expressing marker genes in all three germ layers. Moreover, according to the in vivo analysis, these cells contributed to the formation of teratoma and development of chimeric mice with Mus musculus. Overall, the M. minutoides iPSCs generated in this study possess properties that are comparable to or closely resemble those of naïve pluripotent stem cells (PSCs). These findings suggest these iPSCs have potential utility in various analytical applications, including methods for blastocyst completion.
小耳鼩是世界上最小的哺乳动物之一；然而，其矮小症的调节机制尚未被研究。因此，我们旨在使用 PiggyBac 转座子系统建立小耳鼩诱导多能干细胞 (iPSC)，用于发育工程的应用。所建立的小耳鼩 iPSC 表达多能性标志物，并能分化为神经元。基于体外分化分析，小耳鼩 iPSC 形成了胚状体，在所有三个胚层中表达标记基因。此外，根据体内分析，这些细胞有助于形成畸胎瘤和嵌合小鼠的形成。总的来说，本研究中产生的小耳鼩 iPSC 具有与原始多能干细胞 (PSC) 相当或相似的特性。这些发现表明这些 iPSC 在各种分析应用中具有潜在的用途，包括囊胚完成的方法。
