Vore Andrew S, Marsland Paige, Barney Thaddeus M, Varlinskaya Elena I, Landin Justine D, Healey Kati L, Kibble Sandra, Swartzwelder H S, Chandler Lawrence J, Deak Terrence
Developmental Exposure Alcohol Research Center, Binghamton University-SUNY, Binghamton, NY, United States.
Department of Neurosciences, Charleston Alcohol Research Center, Medical University of South Carolina, Charleston, SC, United States.
Front Physiol. 2024 Jan 24;15:1285376. doi: 10.3389/fphys.2024.1285376. eCollection 2024.
Early initiation of alcohol use during adolescence, and adolescent binge drinking are risk factors for the development of alcohol use disorder later in life. Adolescence is a time of rapid sex-dependent neural, physiological, and behavioral changes as well as a period of heightened vulnerability to many effects of alcohol. The goal of the present studies was to determine age-related changes in blood (leukocyte populations) and body composition across adolescence and early adulthood, and to investigate whether adolescent intermittent ethanol (AIE) exposure would alter the trajectory of adolescent development on these broad physiological parameters. We observed significant ontogenetic changes in leukocyte populations that were mirrored by an age-related increase in cytokine expression among mixed populations of circulating leukocytes. Despite these developmental changes, AIE did not significantly alter overall leukocyte numbers or cytokine gene expression. However, AIE led to sex-specific changes in body fat mass and fat percentage, with AIE-exposed male rats showing significantly decreased fat levels and female rats showing significantly increased fat levels relative to controls. These changes suggest that while AIE may not alter overall leukocyte levels, more complex phenotypic changes in leukocyte populations could underlie previously reported differences in cytokine expression. Coupled with long-term shifts in adipocyte levels, this could have long-lasting effects on innate immunity and the capacity of individuals to respond to later immunological and physiological threats.
青春期过早开始饮酒以及青少年狂饮是日后发生酒精使用障碍的危险因素。青春期是一个神经、生理和行为迅速发生性别依赖性变化的时期，也是对酒精的许多影响高度敏感的时期。本研究的目的是确定青春期和成年早期血液（白细胞群体）和身体成分随年龄的变化，并研究青少年间歇性乙醇（AIE）暴露是否会改变这些广泛生理参数上的青少年发育轨迹。我们观察到白细胞群体存在显著的个体发育变化，循环白细胞混合群体中细胞因子表达随年龄增长而增加反映了这一变化。尽管有这些发育变化，但AIE并没有显著改变白细胞总数或细胞因子基因表达。然而，AIE导致了体脂量和脂肪百分比的性别特异性变化，与对照组相比，暴露于AIE的雄性大鼠脂肪水平显著降低，而雌性大鼠脂肪水平显著升高。这些变化表明，虽然AIE可能不会改变白细胞的总体水平，但白细胞群体中更复杂的表型变化可能是先前报道的细胞因子表达差异的基础。再加上脂肪细胞水平的长期变化，这可能对先天免疫以及个体应对后期免疫和生理威胁的能力产生持久影响。
