Manlapig Jamal James D, Kawakami Suzuna, Matamura Masaya, Kondo Makoto, Ban-Tokuda Tomomi, Matsui Hiroki
Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines.
Graduate School of Bioresources, Mie University, Tsu, Japan.
Anim Sci J. 2024 Jan-Dec;95(1):e13923. doi: 10.1111/asj.13923.
This study was done to investigate which components of rice bran (RB) are involved in the inhibition of methanogenesis by fractionating the rice bran and adding it to a rumen in vitro culture system. The RB extract obtained using ethanol and water was screened in an in vitro fermentation system. The experimental treatment conditions were as follows: a control group containing a substrate without supplements; substrates with 0.06 g of RB; 0.6 mL of ethanol; 0.6 mL of distilled water (DW); 0.6 mL of ethanol-soluble fraction (ESF); 0.06 g of ethanol-insoluble rice bran (EIRB); 0.6 mL of water-soluble fraction (WSF); and 0.06 g of water-insoluble rice bran (WIRB). Based on the result of the analysis, the addition of ESF significantly decreased CH and CH /g dry matter digested, methanogen population (p < 0.05), while gas and dry matter digestibility (DMD) were comparable with the control group. Total short-chain fatty acid (SCFA), and proportion of propionate were reduced, and the proportion of butyrate was increased by the addition of ethanol and ESF (p < 0.05). This result suggests that the supplementation of 10% ESF can substantially reduce methane production in vitro without a negative effect on substrate digestibility.
本研究旨在通过对米糠进行分级并将其添加到瘤胃体外培养系统中，来探究米糠（RB）的哪些成分参与了对甲烷生成的抑制作用。使用乙醇和水获得的米糠提取物在体外发酵系统中进行筛选。实验处理条件如下：一个对照组，包含无添加物的底物；添加0.06克米糠的底物；0.6毫升乙醇；0.6毫升蒸馏水（DW）；0.6毫升乙醇可溶级分（ESF）；0.06克乙醇不溶性米糠（EIRB）；0.6毫升水溶性级分（WSF）；以及0.06克水不溶性米糠（WIRB）。基于分析结果，添加ESF显著降低了每克消化干物质的CH和CH 、产甲烷菌数量（p < 0.05），而气体和干物质消化率（DMD）与对照组相当。添加乙醇和ESF会使总短链脂肪酸（SCFA）以及丙酸比例降低，丁酸比例升高（p < 0.05）。该结果表明，添加10%的ESF可在体外显著降低甲烷产量，且对底物消化率无负面影响。
