Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines.
Graduate School of Bioresources, Mie University, Tsu, Mie, Japan.
Anim Sci J. 2024 Jan-Dec;95(1):e13955. doi: 10.1111/asj.13955.
This study was conducted to assess the effects of fermented rice bran (FRB) with Ligilactobacillus equi on ruminal fermentation using an in vitro system. Oat hay, corn starch, and wheat bran were used as substrate for control. Ten percent of wheat bran was replaced with rice bran (RB), rice bran fermented with distilled water, and rice bran fermented with L. equi for T, T, and T, respectively. The experimental diets were mixed with buffered rumen fluid from wethers under nitrogen gas and incubated for 24 h at 39°C. The fermentation profile and microbial population were analyzed after the incubations. The results revealed that the RB and FRB (with or without L. equi) significantly reduced the gas, methane (CH), and CH per dry matter digested (p < 0.001). Total short-chain fatty acid was also reduced in T and T in comparison with the control (p < 0.001). Propionate proportion was increased while butyrate proportion was reduced in response to treatment addition in cultures (p < 0.001). Anaerobic fungi and Fibrobacter succinogenes abundance were decreased in treatments (p < 0.001). Overall, CH production in vitro can be reduced by RB and FRB supplementation as a result of the reduction of fiber-degrading microorganisms and a decrease in gas production.
本研究旨在评估用嗜酸乳杆菌发酵米糠（FRB）对体外瘤胃发酵的影响。以燕麦干草、玉米淀粉和麦麸作为对照的底物。用米糠（RB）、用蒸馏水发酵的米糠和用嗜酸乳杆菌发酵的米糠分别替代 10%的麦麸，用于 T、T 和 T。实验饲料在氮气下与来自绵羊的缓冲瘤胃液混合，并在 39°C 下孵育 24 小时。孵育后分析发酵特性和微生物种群。结果表明，RB 和 FRB（有或没有嗜酸乳杆菌）显著降低了气体、甲烷（CH）和每干物质消化的 CH（p<0.001）。与对照相比，T 和 T 中的总短链脂肪酸也减少（p<0.001）。丙酸比例增加，而丁酸比例在培养物中因添加处理而减少（p<0.001）。处理降低了厌氧真菌和纤维分解菌的丰度（p<0.001）。总的来说，RB 和 FRB 的添加可以减少纤维分解微生物的数量，减少气体的产生，从而减少体外 CH 的产生。
