Lombardo Marco, Cesareo Massimo, Falsini Benedetto, Cusumano Andrea
Department of Experimental Medicine, Ophthalmology Unit, University of Rome Tor Vergata, 00133 Rome, Italy.
Macula & Genoma Foundation, 00133 Rome, Italy.
Diagnostics (Basel). 2024 Jan 24;14(3):253. doi: 10.3390/diagnostics14030253.
This work aims to reveal the microscopic (2-3 micrometer resolution) appearance of human myelinated nerve fibers in vivo for the first time. We analyzed the myelinated retinal nerve fibers of a male patient without other neurological disorders in a non-invasive way using the transscleral optical phase imaging method with adaptive optics. We also analyzed the fellow eye with non-myelinated nerve fibers and compared the results with traditional ocular imaging methods such as optical coherence tomography. We documented the microscopic appearance of human myelin and myelinated axons in vivo. This method allowed us to obtain better details than through traditional ocular imaging methods. We hope these findings will be useful to the scientific community to evaluate neuro-retinal structures through new imaging techniques and more accurately document nerve anatomy and the pathophysiology of this disease.
这项工作旨在首次揭示人类有髓神经纤维在体内的微观（2-3微米分辨率）外观。我们使用具有自适应光学的经巩膜光学相位成像方法，以非侵入性方式分析了一名无其他神经系统疾病的男性患者的有髓视网膜神经纤维。我们还分析了另一只具有无髓神经纤维的眼睛，并将结果与传统眼部成像方法（如光学相干断层扫描）进行了比较。我们记录了人类髓磷脂和有髓轴突在体内的微观外观。与传统眼部成像方法相比，这种方法使我们能够获得更好的细节。我们希望这些发现将有助于科学界通过新的成像技术评估神经视网膜结构，并更准确地记录神经解剖结构和该疾病的病理生理学。
