Tao Ran, Liu Yaqiu, Chen Su, Shityakov Sergey
College of Computer and Control Engineering, Northeast Forestry University, Harbin 150040, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Plants (Basel). 2024 Jan 23;13(3):337. doi: 10.3390/plants13030337.
The bZIP (basic leucine zipper) transcription factors have been identified as key regulators of plant responses to drought stress, which limits plant growth and yield. Overexpression of bZIP genes has shown potential in enhancing drought tolerance in various plant species. However, the constrained types of individual studies and inconsistencies among experimental approaches has resulted in a lack of statistical significance and limited the extrapolation of bZIP transcription factor overexpression for plant improvement. We conducted a meta-analysis to evaluate ten measured parameters of drought tolerance in bZIP transcription factor-expressing plants as well as moderators affecting the performance of transgenic plants. The results showed that seven parameters, including survival rate as well as the content of regulatory substances (proline accumulation, HO concentration, CAT activity, POD activity, SOD activity and MDA accumulation), were most affected while the impact on physiological status indicators is not significant. In addition, donor/recipient species, treatment medium, duration and methods of simulating drought stress all significantly impacted the degree of drought stress tolerance in plants to some extent among the considered moderators. The findings underscore the potential of bZIP transcription factors as key targets for genetic engineering approaches aimed at improving plant resilience to water scarcity.
bZIP（碱性亮氨酸拉链）转录因子已被确定为植物对干旱胁迫响应的关键调节因子，干旱胁迫会限制植物生长和产量。bZIP基因的过表达已显示出在增强各种植物物种耐旱性方面的潜力。然而，个别研究类型受限以及实验方法之间的不一致导致缺乏统计学意义，并限制了bZIP转录因子过表达在植物改良中的推广应用。我们进行了一项荟萃分析，以评估表达bZIP转录因子的植物中十个耐旱性测量参数以及影响转基因植物性能的调节因素。结果表明，包括存活率以及调节物质含量（脯氨酸积累、HO浓度、CAT活性、POD活性、SOD活性和MDA积累）在内的七个参数受影响最大，而对生理状态指标的影响不显著。此外，在考虑的调节因素中，供体/受体物种、处理介质、模拟干旱胁迫的持续时间和方法在一定程度上均对植物的耐旱胁迫程度有显著影响。这些发现强调了bZIP转录因子作为旨在提高植物对缺水耐受性的基因工程方法关键靶点的潜力。
