Zheng Gang, Wang Dongxing, Mao Kai, Wang Musen, Wang Jian, Xun Wenjuan, Huang Shuai
Forage Processing and Ruminant Nutrition Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Animals (Basel). 2024 Jan 28;14(3):425. doi: 10.3390/ani14030425.
The critical role of the rumen microbiota in the growth performance of livestock is recognized, yet its significance in determining the body weight of goat kids before weaning remains less understood. To bridge this gap, our study delved into the rumen microbiota, serum metabolome, rumen fermentation, and rumen development in goat kids with contrasting body weights before weaning. We selected 10 goat kids from a cohort of 100, categorized into low body weight (LBW, 5.56 ± 0.98 kg) and high body weight (HBW, 9.51 ± 1.01 kg) groups. The study involved sampling rumen contents, tissues, and serum from these animals. Our findings showed that the HBW goat kids showed significant enrichment of VFA-producing bacteria, particularly microbiota taxa within the genera (, , and ) and the genus. This enrichment correlated with elevated acetate and butyrate levels, positively influencing rumen papillae development. Additionally, it was associated with elevated serum levels of glucose, total cholesterol, and triglycerides. The serum metabonomic analysis revealed marked differences in fatty acid metabolism between the LBW and HBW groups, particularly in encompassing oleic acid and both long-chain saturated and polyunsaturated fatty acids. Further correlational analysis underscored a significant positive association between and specific lipids, such as phosphatidylcholine (PC) (22:5/18:3) and PC (20:3/20:1) (r > 0.60, < 0.05). In summary, this study underscores the pivotal role of the rumen microbiota in goat kids' weight and its correlation with specific serum metabolites. These insights could pave the way for innovative strategies aimed at improving animal body weight through targeted modulation of the rumen microbiota.
瘤胃微生物群在牲畜生长性能中的关键作用已得到认可，但其在断奶前山羊羔体重决定中的重要性仍鲜为人知。为填补这一空白，我们的研究深入探讨了断奶前体重不同的山羊羔的瘤胃微生物群、血清代谢组、瘤胃发酵和瘤胃发育情况。我们从100只山羊羔的群体中挑选了10只，分为低体重（LBW，5.56±0.98千克）和高体重（HBW，9.51±1.01千克）组。该研究涉及从这些动物身上采集瘤胃内容物、组织和血清样本。我们的研究结果表明，高体重山羊羔显示出产生挥发性脂肪酸的细菌显著富集，特别是属（、和）和属内的微生物分类群。这种富集与乙酸盐和丁酸盐水平升高相关，对瘤胃乳头发育有积极影响。此外，它还与血清葡萄糖、总胆固醇和甘油三酯水平升高有关。血清代谢组学分析显示，低体重组和高体重组之间在脂肪酸代谢方面存在显著差异，特别是在油酸以及长链饱和脂肪酸和多不饱和脂肪酸方面。进一步的相关性分析强调了与特定脂质之间存在显著正相关，如磷脂酰胆碱（PC）（22:5/18:3）和PC（20:3/20:1）（r>0.60，<0.05）。总之，本研究强调了瘤胃微生物群在山羊羔体重中的关键作用及其与特定血清代谢物的相关性。这些见解可为旨在通过有针对性地调节瘤胃微生物群来提高动物体重的创新策略铺平道路。
