College of Coastal Agricultural Science, Guangdong Ocean University, Zhanjiang 524088, China.
South China Branch of National Saline-Alkali Tolerant Rice Technology Innovation Center, Zhanjiang 524088, China.
Int J Mol Sci. 2024 Feb 1;25(3):1802. doi: 10.3390/ijms25031802.
Nitrogen fixation, occurring through the symbiotic relationship between legumes and rhizobia in root nodules, is crucial in sustainable agriculture. Nodulation and soybean production are influenced by low levels of phosphorus stress. In this study, we discovered a MADS transcription factor, , which is preferentially expressed in nodules and displays significantly increased expression under conditions of phosphate (Pi) deficiency. The overexpression of in composite transgenic plants resulted in an increased number of nodules, higher fresh weight, and enhanced soluble Pi concentration, which subsequently increased the nitrogen content, phosphorus content, and overall growth of soybean plants. Additionally, transcriptome analysis revealed that the overexpression of significantly upregulated the expression of genes associated with nodule growth, such as , , , , and . Based on these findings, we concluded that likely participates in the phosphorus signaling pathway and positively regulates nodulation in soybeans. The findings of this research may lay the theoretical groundwork for further studies and candidate gene resources for the genetic improvement of nutrient-efficient soybean varieties in acidic soils.
氮固定是通过豆科植物和根瘤菌在根瘤中的共生关系来实现的，对可持续农业至关重要。结瘤和大豆生产受到低磷胁迫的影响。在这项研究中，我们发现了一个 MADS 转录因子 ，它在根瘤中优先表达，并在磷酸盐（Pi）缺乏的条件下表达显著增加。在复合转基因植物中过表达 导致根瘤数量增加、鲜重增加和可溶性 Pi 浓度提高，从而增加了氮含量、磷含量和大豆植物的整体生长。此外，转录组分析表明， 过表达显著上调了与根瘤生长相关的基因的表达，如 、 、 、 和 。基于这些发现，我们得出结论， 可能参与磷信号通路，并正向调节大豆中的结瘤。这项研究的结果可能为进一步研究和候选基因资源奠定理论基础，以改善酸性土壤中养分高效型大豆品种的遗传特性。
