Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China.
Plant Sci. 2022 Jul;320:111283. doi: 10.1016/j.plantsci.2022.111283. Epub 2022 Apr 11.
Purple acid phosphatase (PAP) is an important plant acid phosphatase, which can secrete to the rhizosphere to decompose organophosphorus, promote phosphorus use efficiency, plant growth and development. However, little is known about the functions of intracellular PAP in plants, especially for soybean. Our previous study integrating QTL mapping and transcriptome analysis identified an promising low phosphorus (LP)-induced gene GmPAP17. Here, we determined that GmPAP17 was mainly expressed in roots and had a strong response to LP stress. Furthermore, and the relative expression in the root of LP tolerant genotypes NN94-156 was significantly greater than that of LP sensitive genotype Bogao after LP stress treatment. The overexpression of GmPAP17 significantly enhanced both acid phosphatase activity and growth performance of hairy roots under LP stress condition, it was vice versa for RNAi interference of GmPAP17, indicating that GmPAP17 plays an important role in P use efficiency. Moreover, yeast two-hybrid and bimolecular fluorescence complementation analysis showed that GmRAP2.2 was involved in the regulation network of GmPAP17. Taken together, our results suggest that GmPAP17 is a novel plant PAP that functions in the adaptation of soybean to LP stress, possibly through its involvement in P recycling in plants.
紫色酸性磷酸酶（PAP）是一种重要的植物酸性磷酸酶，它可以分泌到根际，分解有机磷，促进磷的利用效率、植物生长和发育。然而，人们对植物细胞内 PAP 的功能知之甚少，特别是对大豆。我们之前的研究将 QTL 作图和转录组分析相结合，鉴定了一个有希望的低磷（LP）诱导基因 GmPAP17。在这里，我们确定 GmPAP17 主要在根中表达，并对 LP 胁迫有强烈的反应。此外，LP 耐受基因型 NN94-156 的根中的相对表达在 LP 胁迫处理后明显高于 LP 敏感基因型 Bogao。GmPAP17 的过表达显著增强了酸性磷酸酶活性和根毛在 LP 胁迫条件下的生长性能，而 GmPAP17 的 RNAi 干扰则相反，表明 GmPAP17 在磷利用效率中起着重要作用。此外，酵母双杂交和双分子荧光互补分析表明，GmRAP2.2 参与了 GmPAP17 的调控网络。总之，我们的研究结果表明，GmPAP17 是一种新的植物 PAP，它在大豆适应 LP 胁迫中起作用，可能通过参与植物中的磷循环。
