Jahan Taskin, Saleh Ahmed Abu, Anwar Shaheda
Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh.
Int J Rheumatol. 2024 Feb 2;2024:3728179. doi: 10.1155/2024/3728179. eCollection 2024.
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that involves cytokines in its pathogenesis. This study is aimed at investigating if gene polymorphisms in cytokines like IL-17, IL-4, IL-6, and IL-12 affect RA susceptibility and severity in the Bangladeshi population. This was a cross-sectional comparative study that included 40 diagnosed RA patients according to the American College of Rheumatology (ACR) criteria 2010, who were free from other rheumatological diseases, and 40 healthy subjects for comparison. The study used PCR-RFLP to determine the IL-17, IL-4, IL-6, and IL-12 cytokine gene polymorphisms. Patients had a mean age of 37.22 ± 6.70 years. Among the patients, 31 were female and 9 were male. The mean disease duration was 18.11 ± 7.39 months. The study found that rheumatoid arthritis patients with the IL-17F (7488 A/G) polymorphism with GG genotype ( = 0.006, OR = 8.56, 95% CI = 1.77 - 41.33) and IL-12B (1188 A/C) polymorphism with AC ( = 0.012, OR = 3.69, 95% CI = 1.43 - 9.53) and CC ( = 0.013, OR = 7.58, 95% CI = 1.56 - 36.88) genotypes were significantly associated with disease risk. Furthermore, patients with the IL-17F (7488) GG genotype and IL-12B (1188) AC and CC genotypes had higher rheumatoid arthritis disease severity and activity parameters. The study found no significant association between polymorphisms involving IL-4 (590 C/T) and IL-6 (174 G/C) genes and rheumatoid arthritis disease risk in the Bangladeshi population. Gene polymorphisms in cytokines IL-17F (7488 A/G) and IL-12B (1188 A/C) can predict disease susceptibility and severity in Bangladeshi rheumatoid arthritis patients.
类风湿性关节炎（RA）是一种慢性自身免疫性炎症疾病，其发病机制涉及细胞因子。本研究旨在调查白细胞介素-17（IL-17）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）和白细胞介素-12（IL-12）等细胞因子的基因多态性是否会影响孟加拉人群中RA的易感性和严重程度。这是一项横断面比较研究，根据2010年美国风湿病学会（ACR）标准纳入了40例确诊的RA患者，这些患者无其他风湿性疾病，并纳入40名健康受试者作为对照。该研究使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）来确定IL-17、IL-4、IL-6和IL-12细胞因子基因多态性。患者的平均年龄为37.22±6.70岁。患者中，女性31例，男性9例。平均病程为18.11±7.39个月。研究发现，具有GG基因型的IL-17F（7488 A/G）多态性（P = 0.006，比值比[OR]=8.56，95%置信区间[CI]=1.77 - 41.33）以及具有AC（P = 0.012，OR = 3.69，95% CI = 1.43 - 9.53）和CC（P = 0.013，OR = 7.58，95% CI = 1.56 - 36.88）基因型的IL-12B（1188 A/C）多态性与疾病风险显著相关。此外，具有IL-17F（7488）GG基因型以及IL-12B（1188）AC和CC基因型的患者具有更高的类风湿性关节炎疾病严重程度和活动参数。该研究发现，在孟加拉人群中，涉及IL-4（590 C/T）和IL-6（174 G/C）基因的多态性与类风湿性关节炎疾病风险之间无显著关联。细胞因子IL-17F（7488 A/G）和IL-12B（1188 A/C）的基因多态性可预测孟加拉类风湿性关节炎患者的疾病易感性和严重程度。
