Clin Lab. 2024 Feb 1;70(2). doi: 10.7754/Clin.Lab.2023.230821.
Seoul virus (SEOV) is a significant causative pathogen of hemorrhagic fever with renal syndrome (HFRS). Accurate discrimination of SEOV infection from other viral or bacterial infections holds vital clinical importance.
Our study utilized quantitative real-time PCR (qRT-PCR), metagenomic next-generation sequencing (mNGS), and immunological assays to identify the pathogen causing HFRS.
For the case, mNGS identified SEOV and suspected host or environmental microorganisms at 5 days from symptom onset. qRT-PCR detected SEOV between 5 to 8 days from symptom onset. Anti-hantavirus IgM antibodies reached positive criteria at 7 days and IgG antibodies at 9 days from symptom onset.
qRT-PCR, mNGS, and immunological assays each have merits and drawbacks. Optimal selection depends on laboratory conditions and clinical requirements.
汉城病毒（SEOv）是肾综合征出血热（HFRS）的重要病原体。准确区分 SEOV 感染与其他病毒或细菌感染具有重要的临床意义。
本研究采用实时定量 PCR（qRT-PCR）、宏基因组下一代测序（mNGS）和免疫检测方法来确定引起 HFRS 的病原体。
在该病例中，mNGS 在发病后 5 天检测到 SEOV 和疑似宿主或环境微生物。qRT-PCR 在发病后 5 至 8 天检测到 SEOV。抗汉坦病毒 IgM 抗体在发病后 7 天达到阳性标准，IgG 抗体在发病后 9 天达到阳性标准。
qRT-PCR、mNGS 和免疫检测各有优缺点。最佳选择取决于实验室条件和临床需求。
