Department of Chemistry, School of Science, Kitasato University, Sagamihara, Japan.
Methods Mol Biol. 2024;2763:3-36. doi: 10.1007/978-1-0716-3670-1_1.
A mucin-type glycoprotein extracted from various species of jellyfish (JF) is named qniumucin (Q-mucin). Compared with general mucins, most of which are from mammals including humans, Q-mucin can be collected on a relatively large scale with high yield. Owing to its simple structure with low heterogeneity, Q-mucin has a potential to be developed into material mucins which opens various applications valuable to humans. On the basis of our present knowledge, here, we describe our protocol for the extraction of Q-mucin, which can be extracted from any JF species worldwide. Experimental protocols to identify the structure of Q-mucin are also introduced.
从各种水母（JF）中提取的一种粘蛋白型糖蛋白被命名为 qniumucin（Q-粘蛋白）。与通常的粘蛋白（主要来自包括人类在内的哺乳动物）相比，Q-粘蛋白可以在相对较大的规模上以高产率收集。由于其结构简单、异质性低，Q-粘蛋白具有开发成对人类有价值的材料粘蛋白的潜力。基于我们目前的知识，在这里，我们描述了从世界上任何一种水母物种中提取 Q-粘蛋白的提取方法。我们还介绍了鉴定 Q-粘蛋白结构的实验方案。
