Eco-Soft Materials Research Unit, Advanced Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Carbohydr Res. 2009 Nov 2;344(16):2182-7. doi: 10.1016/j.carres.2009.08.001. Epub 2009 Aug 7.
The structure of O-glycan in qniumucin (Q-mucin), which is a novel mucin extracted from jellyfish, was analyzed by a combination of NMR and ESI-MS/MS. A previously unidentified monosaccharide involved in the glycan chains was determined to be N-acetylgalactosamine (GalNAc) substituted by 2-aminoethylphosphonate (AEP) at the C-6. The O-glycans in Q-mucin from Aurelia aurita were proved to be mainly composed of three monosaccharides: GalNAc, AEP-(O-->6)-GalNAc, and P-6-GalNAc. To the best of our knowledge, this is the first example of an O-glycan structure of glycoproteins containing AEP. This exceptionally simple structure of Q-mucin and its potential use in material science and technology are revealed.
从海蜇中提取的新型粘蛋白 qumucin（Q-mucin）的 O-聚糖结构通过 NMR 和 ESI-MS/MS 的组合进行了分析。确定糖链中存在一种以前未识别的单糖，为在 C-6 上被 2-氨基乙基膦酸酯（AEP）取代的 N-乙酰半乳糖胺（GalNAc）。来自 Aurelia aurita 的 Q-mucin 的 O-聚糖被证明主要由三种单糖组成：GalNAc、AEP-（O-->6）-GalNAc 和 P-6-GalNAc。据我们所知，这是含有 AEP 的糖蛋白 O-聚糖结构的第一个例子。揭示了 Q-mucin 异常简单的结构及其在材料科学和技术中的潜在用途。
