Yates J A, Higashi G I
Am J Trop Med Hyg. 1985 Nov;34(6):1132-7. doi: 10.4269/ajtmh.1985.34.1132.
Vaccination of inbred jirds (Meriones unguiculatus) with 60cobalt radiation-attenuated Brugia malayi infective stage larvae (L3) protected against homologous challenge given either subcutaneously (sc) or by the intraperitoneal (ip) route. Groups of jirds vaccinated once sc with 75, 15 Krad L3 showed from 69% to 91% reduction in recovered worms after ip challenge infection compared to infection in non-vaccinated control jirds, while 75% reduction in mean worm burden was seen in jirds receiving sc challenge infection. A single sc vaccination with 75, 10 or 20 Krad L3 produced no protection (10 Krad) and 64% reduction in recovered worms (20 Krad). Therefore the 15 Krad dose appeared to be best. A marked increase in anti-B. malayi antibody in vaccinated jirds was seen (by ELISA) immediately after challenge infection and an immunofluorescence assay showed that L3 incubated in serum from vaccinated jirds were completely and uniformly covered with specific antibody. Eosinophil-rich granulomas containing dead and moribund L3 were recovered from vaccinated jirds. This model of protective immunity in a Brugia-susceptible small rodent may provide a useful system for identification of molecularly defined filarial-protective immunogens.
用60钴辐射减毒的马来布鲁线虫感染期幼虫（L3）对近交系沙鼠（长爪沙鼠）进行接种，可抵御通过皮下（sc）或腹腔内（ip）途径进行的同源攻击。单次皮下接种75、15拉德L3的沙鼠组，与未接种的对照沙鼠相比，腹腔内攻击感染后回收的蠕虫减少了69%至91%，而接受皮下攻击感染的沙鼠平均虫负荷减少了75%。单次皮下接种75、10或20拉德L3，10拉德组无保护作用，20拉德组回收的蠕虫减少了64%。因此，15拉德剂量似乎是最佳的。攻击感染后立即（通过ELISA）观察到接种沙鼠体内抗马来布鲁线虫抗体显著增加，免疫荧光试验表明，在接种沙鼠血清中孵育的L3完全且均匀地被特异性抗体覆盖。从接种沙鼠中回收了含有死亡和濒死L3的富含嗜酸性粒细胞的肉芽肿。这种在对布鲁线虫易感的小型啮齿动物中的保护性免疫模型可能为鉴定分子明确的丝虫保护性免疫原提供一个有用的系统。
