Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, Brazil.
Department of Aerospace Science and Technology, Institute of Advanced Studies, Technological Institute of Aeronautics (ITA), Praça Marechal Eduardo Gomes, São José dos Campos, SP, Brazil.
J Biomed Mater Res B Appl Biomater. 2024 Feb;112(2):e35380. doi: 10.1002/jbm.b.35380.
Zirconia implants are gaining attention as a viable alternative to titanium implants due to their comparable osseointegration development, improved soft tissue adaptation, and enhanced aesthetics. An encouraging avenue for improving zirconia implant properties involves the potential application of bioactive coatings to their surfaces. These coatings have shown potential for inducing hydroxyapatite formation, crucial for bone proliferation, and improving implant mechanical properties. This study aimed to evaluate the effect of coating zirconia implants with two bioactive glasses, 45S5 and BioK, on osteogenesis in vitro and osseointegration in vivo. Zirconia samples and implants were prepared using Zpex zirconia powder and blocks, respectively. The samples were divided into three groups: polished zirconia (ZRC), zirconia coated with 45S5 bioglass (Z + 45S5), and zirconia coated with BioK glass (Z + BK). Coatings were applied using a brush and sintered at 1200°C. Chemical analysis of the coatings was carried out using x-ray diffraction and Fourier Transform Infrared Spectroscopy. Surface topography and roughness were characterized using scanning electron microscopy and a roughness meter. In vitro experiments used mesenchymal cells from Wistar rat femurs, and the coated zirconia implants were found to promote cell viability, protein synthesis, alkaline phosphatase activity, and mineralization, indicating enhanced osteogenesis. In vivo experiments with 18 rats showed positive results for bone formation and osseointegration through histological and histomorphometric analysis and a push-out test. The findings indicate that bioactive glass coatings have the potential to improve cell differentiation, bone formation, and osseointegration in zirconia implants.
氧化锆植入物因其可比的骨整合发展、改善的软组织适应性和增强的美学效果，正作为钛植入物的可行替代品而受到关注。改善氧化锆植入物性能的一个令人鼓舞的途径是在其表面应用生物活性涂层。这些涂层在诱导羟基磷灰石形成方面显示出潜力，这对骨增殖和改善植入物机械性能至关重要。本研究旨在评估用两种生物活性玻璃（45S5 和 BioK）涂覆氧化锆植入物对体外成骨和体内骨整合的影响。使用 Zpex 氧化锆粉末和块分别制备氧化锆样品和植入物。将样品分为三组：抛光氧化锆（ZRC）、涂覆 45S5 生物玻璃的氧化锆（Z + 45S5）和涂覆 BioK 玻璃的氧化锆（Z + BK）。使用刷子施加涂层并在 1200°C 下烧结。使用 X 射线衍射和傅里叶变换红外光谱对涂层进行化学分析。使用扫描电子显微镜和粗糙度计对表面形貌和粗糙度进行了表征。体外实验使用来自 Wistar 大鼠股骨的间充质细胞，发现涂覆的氧化锆植入物可促进细胞活力、蛋白质合成、碱性磷酸酶活性和矿化，表明成骨作用增强。18 只大鼠的体内实验通过组织学和组织形态计量学分析和推出试验显示出骨形成和骨整合的积极结果。研究结果表明，生物活性玻璃涂层有可能改善氧化锆植入物中的细胞分化、骨形成和骨整合。
