Bowers Robert M, Gonzalez-Pena Veronica, Wardhani Kartika, Goudeau Danielle, Blow Matthew James, Udwary Daniel, Klein David, Vill Albert C, Brito Ilana L, Woyke Tanja, Malmstrom Rex, Gawad Charles
DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Department of Pediatrics, Stanford University, Stanford, CA, USA.
bioRxiv. 2024 Jan 31:2024.01.30.577819. doi: 10.1101/2024.01.30.577819.
Microbial genomes produced by single-cell amplification are largely incomplete. Here, we show that primary template amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard amplification approaches were usually <30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters.
通过单细胞扩增产生的微生物基因组大多是不完整的。在此，我们表明，一种新型的单细胞扩增技术——初级模板扩增（PTA），能够从三种细菌分离物种中生成近乎完整的基因组。此外，使用PTA从水生和土壤微生物群落中获得的分类学上多样化的基因组，其完整性中位数为81%，而采用标准扩增方法获得的基因组通常完整性低于30%。源自PTA的基因组还包含更多相关病毒和生物合成基因簇。
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