Wang Yulan, Wu Jing, Zhao Jian, Xu Tianyi, Zhang Meng, Liu Jingjing, Wang Yixuan, Wang Quan, Song Xiaofeng
Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.
School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, China.
Mol Ther Nucleic Acids. 2024 Jan 24;35(1):102127. doi: 10.1016/j.omtn.2024.102127. eCollection 2024 Mar 12.
RNA editing plays an extensive role in the initiation and progression of cancer. However, the overall profile and molecular functions of RNA editing in different ovarian cancer subtypes have not been fully characterized and elucidated. Here, we conducted a study on RNA editing in four cohorts of ovarian cancer subtypes through large-scale parallel reporting and bioinformatics analysis. Our findings revealed that RNA editing patterns exhibit subtype-specific characteristics within cancer subtypes. The expression pattern of ADAR and the number of differential editing sites varied under different conditions. CCOC and EOC exhibited significant editing deficiency, whereas HGSC and MOC displayed significant editing excess. The sites within the turquoise module of the coedited network also revealed their correlation with ovarian cancer. In addition, we identified an average of over 40,000 -edQTLs in the four subtypes. Finally, we explored the association between RNA editing and drug response, uncovering several potentially effective editing-drug pairs (EDP) and suggesting the conceivable utility of RNA editing sites as therapeutic targets for cancer treatment. Overall, our comprehensive study has identified and characterized RNA editing events in various subtypes of ovarian cancer, providing a new perspective for ovarian cancer research and facilitating the development of medical interventions and treatments.
RNA编辑在癌症的发生和发展中发挥着广泛作用。然而，不同卵巢癌亚型中RNA编辑的整体概况和分子功能尚未得到充分表征和阐明。在此，我们通过大规模平行报告和生物信息学分析，对四组卵巢癌亚型的RNA编辑进行了研究。我们的研究结果表明，RNA编辑模式在癌症亚型中呈现出亚型特异性特征。ADAR的表达模式和差异编辑位点的数量在不同条件下有所不同。浆液性癌（CCOC）和子宫内膜样癌（EOC）表现出显著的编辑缺陷，而高级别浆液性癌（HGSC）和黏液性癌（MOC）则表现出显著的编辑过剩。共编辑网络绿松石模块中的位点也揭示了它们与卵巢癌的相关性。此外，我们在四种亚型中平均鉴定出超过40,000个编辑数量性状位点（edQTL）。最后，我们探讨了RNA编辑与药物反应之间的关联，发现了几种潜在有效的编辑-药物对（EDP），并表明RNA编辑位点作为癌症治疗的治疗靶点具有潜在效用。总体而言，我们的综合研究已鉴定并表征了各种卵巢癌亚型中的RNA编辑事件，为卵巢癌研究提供了新视角，并促进了医学干预和治疗的发展。
