Chaudhary Mridul Kant, Kumar Bhanu, Agnihotri Priyanka, Srivastava Sharad
Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow, India.
Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute, Lucknow, India.
Nat Prod Res. 2024 Feb 14:1-6. doi: 10.1080/14786419.2024.2314634.
The genus has high significance in Indian and other traditional systems of medicine due to the presence of -quinoline alkaloids. This study was conducted to record the metabolic variation in eight spp. collected from the Western Himalayan region. The RP-HPLC-PDA chromatogram separates the markers jatrorrhizine, palmatine and berberine at specific retention time, 11.45, 16.41 and 18.15 min respectively. The method was validated on linearity, precision and recovery indices as per International Conference on Harmonisation (ICH) guidelines. The maximum content of berberine, palmatine and jatrorrhizine was found in (1.74% ± 0.032, 1.63% ± 0.028 and 0.264% ± 0.012, respectively) on % dry weight basis. This study will help in phyto-marker-based identification and differentiation of various species, which can be used as an alternative to the official drug i.e. .
由于含有喹啉生物碱，该属植物在印度和其他传统医学体系中具有重要意义。本研究旨在记录从喜马拉雅西部地区采集的8种该属植物的代谢差异。反相高效液相色谱-光电二极管阵列（RP-HPLC-PDA）色谱图在特定保留时间分别为11.45、16.41和18.15分钟时分离出小檗碱、巴马汀和黄连碱等标志物。该方法根据国际协调会议（ICH）指南在线性、精密度和回收率指标方面进行了验证。以干重百分比计，在某植物中发现小檗碱、巴马汀和黄连碱的含量最高（分别为1.74%±0.032、1.63%±0.028和0.264%±0.012）。本研究将有助于基于植物标志物对各种该属植物进行鉴定和区分，这可作为官方药物即某药物的替代品。
