Evaluation of -quinoline alkaloids by RP-HPLC-PDA in different species collected from Western Himalayan Region.

The genus has high significance in Indian and other traditional systems of medicine due to the presence of -quinoline alkaloids. This study was conducted to record the metabolic variation in eight spp. collected from the Western Himalayan region. The RP-HPLC-PDA chromatogram separates the markers jatrorrhizine, palmatine and berberine at specific retention time, 11.45, 16.41 and 18.15 min respectively. The method was validated on linearity, precision and recovery indices as per International Conference on Harmonisation (ICH) guidelines. The maximum content of berberine, palmatine and jatrorrhizine was found in (1.74% ± 0.032, 1.63% ± 0.028 and 0.264% ± 0.012, respectively) on % dry weight basis. This study will help in phyto-marker-based identification and differentiation of various species, which can be used as an alternative to the official drug i.e. .