Tibet Autonomous Animal Disease Prevention and Control Center, Lhasa, 850000, Tibet, China.
Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa, 850009, Tibet, China.
Parasitol Res. 2024 Feb 14;123(2):132. doi: 10.1007/s00436-024-08158-5.
To determine the genotypes of the epidemic strains of Echinococcus granulosus in livestock in Tibet, samples of E. granulosus cysts were collected from 11 yaks and 62 sheep. Genomic DNA was extracted from these samples, and gene fragments of mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit I (nad1) were amplified by PCR and sequenced. DNASTAR and MAGA7.0 were employed for homology analysis and phylogenetic tree construction. Echinococcus granulosus cysts were detected in 56.2% (41/73) of the samples screened. Of these, 63.4% (26/41) were identified as E. granulosus G1 genotype (common sheep strain), 24.4% (10 /41) as G3 genotype (buffalo strain), and 12.2% (5/41) were G6 genotype (camel strain). The study concludes that yaks and sheep in Langkazi county, Tibet, carry three E. granulosus genotypes (G1, G3, and G6), with the G1 genotype the predominant genotype in the region. This study clarifies the distribution of E. granulosus genotypes, providing genetic data and insight for the surveillance and prevention of echinococcosis.
为了确定西藏家畜流行细粒棘球蚴虫株的基因型，从 11 头牦牛和 62 只绵羊中采集了细粒棘球蚴囊包样本。从这些样本中提取基因组 DNA，通过 PCR 扩增和测序，扩增线粒体细胞色素 c 氧化酶亚基 I (cox1)和 NADH 脱氢酶亚基 I (nad1)的基因片段。使用 DNASTAR 和 MAGA7.0 进行同源性分析和系统发育树构建。在筛选的 73 个样本中，检测到 56.2%（41/73）含有细粒棘球蚴囊包。其中，63.4%（26/41）鉴定为细粒棘球蚴 G1 基因型（普通绵羊株），24.4%（10/41）为 G3 基因型（水牛株），12.2%（5/41）为 G6 基因型（骆驼株）。本研究表明，西藏朗县的牦牛和绵羊携带三种细粒棘球蚴基因型（G1、G3 和 G6），其中 G1 基因型是该地区的主要基因型。本研究阐明了细粒棘球蚴基因型的分布，为包虫病的监测和预防提供了遗传数据和见解。
