Huang Chuanlin, Zhou Wenjuan, Guan Weijiang, Ye Nengsheng
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Anal Chim Acta. 2024 Mar 22;1295:342324. doi: 10.1016/j.aca.2024.342324. Epub 2024 Feb 3.
Luminol chemiluminescence (CL) sensing system remains an excellent candidate for application in bioanalysis due to its good water solubility. However, traditional luminol CL system usually requires the addition of oxidizing agents and sensitizers to obtain high efficiency for the improvement of detection sensitivity. Although numerous studies on the nanomaterial-enhanced luminol CL systems have been carried out, there is still great potential to develop inexpensive, readily available and easily handled catalysts to construct simple and effective CL platform for biomolecular sensing.
Few-layered MoS nanosheets (NS) prepared by sonication-assisted exfoliation of commercially available bulk MoS were found to significantly enhance the CL of luminol‒dissolved oxygen in the absence of additional oxidants. The mechanism study demonstrated that exfoliated MoS NS could catalyze the decomposition of dissolved oxygen by virtue of its exposed active sites on the surface, generating increased reactive oxygen intermediates, which then oxidize luminol and produce intense CL emission. The proposed high-efficiency luminol CL system was then employed for the extremely sensitive identification of dopamine based on the quenching of CL by dopamine. The limit of detection (LOD) for dopamine can be as low as 2.07 nM. Besides, it also works well in the actual urine sample with good recoveries (99.6-100.6 %), confirming the practicability of the method.
As a new type of CL catalyst, MoS NS developed in this work are easy to obtain, simple to prepare and can be produced in large quantities, which lays a foundation for extending applicability of MoS NS in the CL field, and provides a new idea for developing simple and highly sensitive CL sensing system.
鲁米诺化学发光（CL）传感系统因其良好的水溶性，仍是生物分析应用中的优秀候选者。然而，传统的鲁米诺CL系统通常需要添加氧化剂和敏化剂以提高检测灵敏度来获得高效率。尽管已经对纳米材料增强的鲁米诺CL系统进行了大量研究，但开发廉价、易于获得且易于处理的催化剂以构建用于生物分子传感的简单有效的CL平台仍有很大潜力。
通过超声辅助剥离市售块状MoS₂制备的少层MoS₂纳米片（NS）被发现可在不添加额外氧化剂的情况下显著增强鲁米诺 - 溶解氧的CL。机理研究表明，剥离的MoS₂ NS可凭借其表面暴露的活性位点催化溶解氧的分解，产生更多的活性氧中间体，进而氧化鲁米诺并产生强烈的CL发射。然后，基于多巴胺对CL的猝灭作用，将所提出的高效鲁米诺CL系统用于多巴胺的超灵敏检测。多巴胺的检测限（LOD）可低至2.07 nM。此外，它在实际尿液样本中也能很好地工作，回收率良好（99.6 - 100.6%），证实了该方法的实用性。
本工作中开发的MoS₂ NS作为一种新型的CL催化剂，易于获得、制备简单且可大量生产，为扩展MoS₂ NS在CL领域的适用性奠定了基础，并为开发简单且高灵敏度的CL传感系统提供了新思路。
