MRC Translational Immune Discovery Unit, MRC Weatherall Institute of Molecular Medicine, Oxford, OX3 9DS, UK.
Cell Communication Lab, Department of Oncology, University College London Cancer Institute, 72 Huntley Street, London, WC1E 6DD, UK.
Trends Cell Biol. 2024 Oct;34(10):854-864. doi: 10.1016/j.tcb.2024.01.006. Epub 2024 Feb 13.
Metazoan organisms are heterocellular systems composed of hundreds of different cell types, which arise from an isogenic genome through differentiation. Cellular 'plasticity' further enables cells to alter their fate in response to exogenous cues and is involved in a variety of processes, such as wound healing, infection, and cancer. Recent advances in cellular model systems, high-dimensional single-cell technologies, and lineage tracing have sparked a renaissance in plasticity research. Here, we discuss the definition of cell plasticity, evaluate state-of-the-art model systems and techniques to study cell-fate dynamics, and explore the application of single-cell technologies to obtain functional insights into cell plasticity in healthy and diseased tissues. The integration of advanced biomimetic model systems, single-cell technologies, and high-throughput perturbation studies is enabling a new era of research into non-genetic plasticity in metazoan systems.
后生动物是由数百种不同类型的细胞组成的异细胞系统，这些细胞通过分化从同基因基因组中产生。细胞的“可塑性”进一步使细胞能够根据外源性信号改变其命运，并参与各种过程，如伤口愈合、感染和癌症。细胞模型系统、高维单细胞技术和谱系追踪方面的最新进展激发了可塑性研究的复兴。在这里，我们讨论了细胞可塑性的定义，评估了用于研究细胞命运动力学的最先进的模型系统和技术，并探讨了单细胞技术在获得对健康和患病组织中细胞可塑性的功能见解方面的应用。先进的仿生模型系统、单细胞技术和高通量扰动研究的整合正在使后生动物系统中非遗传可塑性的研究进入一个新时代。
