State Key Laboratory of Genetic Resources and Evolution & Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
Sino-Africa Joint Research Center, Chinese Academy of Sciences, Kunming, China.
BMC Genomics. 2024 Feb 14;25(1):177. doi: 10.1186/s12864-024-10070-2.
Prion diseases, also known as transmissible spongiform encephalopathies (TSEs) remain one of the deleterious disorders, which have affected several animal species. Polymorphism of the prion protein (PRNP) gene majorly determines the susceptibility of animals to TSEs. However, only limited studies have examined the variation in PRNP gene in different Nigerian livestock species. Thus, this study aimed to identify the polymorphism of PRNP gene in Nigerian livestock species (including camel, dog, horse, goat, and sheep). We sequenced the open reading frame (ORF) of 65 camels, 31 village dogs and 12 horses from Nigeria and compared with PRNP sequences of 886 individuals retrieved from public databases.
All the 994 individuals were assigned into 162 haplotypes. The sheep had the highest number of haplotypes (n = 54), and the camel had the lowest (n = 7). Phylogenetic tree further confirmed clustering of Nigerian individuals into their various species. We detected five non-synonymous SNPs of PRNP comprising of G9A, G10A, C11G, G12C, and T669C shared by all Nigerian livestock species and were in Hardy-Weinberg Equilibrium (HWE). The amino acid changes in these five non-synonymous SNP were all "benign" via Polyphen-2 program. Three SNPs G34C, T699C, and C738G occurred only in Nigerian dogs while C16G, G502A, G503A, and C681A in Nigerian horse. In addition, C50T was detected only in goats and sheep.
Our study serves as the first to simultaneously investigate the polymorphism of PRNP gene in Nigerian livestock species and provides relevant information that could be adopted in programs targeted at breeding for prion diseases resistance.
朊病毒病，也称为传染性海绵状脑病（TSEs），仍然是一种有害疾病，它影响了几种动物物种。朊病毒蛋白（PRNP）基因的多态性主要决定了动物对 TSEs 的易感性。然而，只有有限的研究检查了不同尼日利亚牲畜物种中 PRNP 基因的变异。因此，本研究旨在确定尼日利亚牲畜物种（包括骆驼、狗、马、山羊和绵羊）中 PRNP 基因的多态性。我们对来自尼日利亚的 65 只骆驼、31 只乡村犬和 12 匹马的开放阅读框（ORF）进行了测序，并将其与从公共数据库中检索到的 886 个人的 PRNP 序列进行了比较。
所有 994 个人被分配到 162 个单倍型中。绵羊的单倍型数量最多（n=54），而骆驼的单倍型数量最少（n=7）。系统发育树进一步证实了尼日利亚个体聚类到各自的物种。我们检测到 PRNP 中的五个非同义 SNP，包括 G9A、G10A、C11G、G12C 和 T669C，这些 SNP 在所有尼日利亚牲畜物种中都存在，并处于 Hardy-Weinberg 平衡（HWE）状态。通过 Polyphen-2 程序，这些五个非同义 SNP 中的氨基酸变化均为“良性”。三个 SNP G34C、T699C 和 C738G 仅在尼日利亚犬中发生，而 C16G、G502A、G503A 和 C681A 仅在尼日利亚马中发生。此外，C50T 仅在山羊和绵羊中检测到。
本研究首次同时调查了尼日利亚牲畜物种中 PRNP 基因的多态性，并提供了可用于针对朊病毒病抗性进行繁殖计划的相关信息。
