Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
Key Laboratory of Pathogen Infection Prevention and Control (Peking Union Medical College), Ministry of Education, Beijing, China.
BMC Med Educ. 2024 Feb 14;24(1):142. doi: 10.1186/s12909-024-05100-2.
Infectious diseases are a serious threat to human especially since the COVID-19 outbreak has proved the importance and urgency of their diagnosis and treatment again. Metagenomic next-generation sequencing (mNGS) has been widely used and recognized in clinical and carried out localized testing in hospitals. Increasing the training of mNGS detection technicians can enhance their professional quality and more effectively realize the application value of the hospital platform.
Based on the initial theoretical understanding and practice of the mNGS platform for localization construction, we have designed a training program to enhance the ability of technicians to detect pathogens by utilizing mNGS, and hence to conduct training practices nationwide.
Until August 30, 2022, the page views of online classes have reached 51,500 times and 6 of offline small-scale training courses have been conducted. A total of 67 trainees from 67 hospitals have participated in the training with a qualified rate of 100%. After the training course, the localization platform of 1 participating hospital has been put into use, 2 have added the mNGS localization platform for admission, among which 3 have expressed strong intention of localization.
This study focuses on the training procedures and practical experience of the project which is the first systematic standardized program of mNGS in the world. It solves the training difficulties in the current industry, and effectively promotes the localization construction and application of mNGS in hospitals. It has great development potential in the future and is worth further promotion.
传染病是对人类的严重威胁，尤其是自 COVID-19 爆发以来，再次证明了诊断和治疗的重要性和紧迫性。宏基因组下一代测序（mNGS）已在临床中得到广泛应用和认可，并在医院进行了局部检测。增加 mNGS 检测技术人员的培训可以提高他们的专业素质，更有效地实现医院平台的应用价值。
基于本地化构建 mNGS 平台的初步理论理解和实践，我们设计了一项培训计划，以增强技术人员利用 mNGS 检测病原体的能力，从而在全国范围内进行培训实践。
截至 2022 年 8 月 30 日，在线课程的页面浏览量已达到 51500 次，已举办了 6 次线下小规模培训课程。共有来自 67 家医院的 67 名学员参加了培训，合格率为 100%。培训课程结束后，1 家参与医院的本地化平台已投入使用，2 家医院新增 mNGS 本地化平台入院，其中 3 家表示强烈的本地化意向。
本研究重点介绍了该项目的培训程序和实践经验，这是全球首个系统标准化的 mNGS 项目。它解决了当前行业中的培训难题，有效推动了 mNGS 在医院的本地化建设和应用。它具有很大的发展潜力，值得进一步推广。
