二氧化钛/金纳米颗粒对黑色素瘤C540细胞的体外声动力治疗研究
A Study of Sonodynamic Therapy of Melanoma C540 Cells in Vitro by Titania/Gold Nanoparticles.
作者信息
Perota Ghazale, Faghani-Eskandarkolaei Parsa, Zahraie Niloofar, Zare Mohammad Hosein, Sattarahmady Naghmeh
机构信息
Department of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
J Biomed Phys Eng. 2024 Feb 1;14(1):43-54. doi: 10.31661/jbpe.v0i0.2310-1674. eCollection 2024 Feb.
BACKGROUND
Sonodynamic Therapy (SDT), a safe and non-invasive strategy in tumor therapy, is in development using novel sono-sensitizers, activated by low-intensity ultrasound radiation. SDT mainly progresses through Reactive Oxygen Species (ROS) generation followed by cell annihilation.
OBJECTIVE
The current study aimed to investigate the effect of ultrasound therapy with titania/gold nanoparticles (NPs) on melanoma cancer.
MATERIAL AND METHODS
In this experimental study, Titania/gold NPs (TGNPs) were synthesized, and their activity was investigated in sonodynamic therapy of a melanoma cancer cell line (C540). SDT was performed at 1.0 W cm and 1.0 MHz for one minute.
RESULTS
The synthesized NPs that comprised gold NPs of <10 nm into titania NPs of <20 nm showed great stability and cytocompatibility. While TGNPs were biocompatible, a remarkable rate of cell ablation was observed upon ultrasound irradiation due to ROS generation.
CONCLUSION
The SDT using TGNPs can be introduced as an alternative and low-cost treatment method for melanoma malignancy.
背景
声动力疗法（SDT）是一种肿瘤治疗中的安全无创策略，目前正在利用新型声敏剂进行研发，这些声敏剂由低强度超声辐射激活。SDT主要通过产生活性氧（ROS）进而导致细胞凋亡来发挥作用。
目的
本研究旨在探究二氧化钛/金纳米颗粒（NPs）超声治疗对黑色素瘤的影响。
材料与方法
在本实验研究中，合成了二氧化钛/金纳米颗粒（TGNPs），并在黑色素瘤细胞系（C540）的声动力治疗中研究了它们的活性。SDT在1.0W/cm和1.0MHz条件下进行1分钟。
结果
合成的纳米颗粒由小于10nm的金纳米颗粒和小于20nm的二氧化钛纳米颗粒组成，具有良好的稳定性和细胞相容性。虽然TGNPs具有生物相容性，但超声照射后由于ROS的产生，观察到显著的细胞消融率。
结论
使用TGNPs的SDT可作为黑色素瘤恶性肿瘤的一种替代且低成本的治疗方法。
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