Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Photodiagnosis Photodyn Ther. 2022 Sep;39:102991. doi: 10.1016/j.pdpdt.2022.102991. Epub 2022 Jun 30.
Nanodrug delivery systems are novel strategies for tumor treatment since delivery of chemotherapy drugs such as paclitaxel (PTX) is associated with substantial challenges due to its poor aqueous solubility. In addition, sonodynamic therapy (SDT) is a promising approach that can increase the uptake, accumulation, and dispersion of desirable amounts of the drugs by activating sonosensitizer and enhancing cell membrane permeability. Herein, gold-paclitaxel nanoparticles (Au-PTX NPs) were synthesized and characterized to evaluate the cytotoxicity toward C540 cancer cells in comparison of free PTX, AuNPs, and AuNPs+free PTX in the absence and presence of ultrasound radiation. Evidence shows that AuNPs have a median diameter size of 95.0 ± 15.4, while the size of Au-PTX NPs is roughly 219.7 ± 40.4 nm. Negative zeta-potential results indicate high stability and good dispersion of nanoparticles. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results revealed that Au-PTX NPs increased the cytotoxicity compared to other treatment groups that ensure the great potential of AuNPs as a promising nano-carrier for PTX drug delivery. Moreover, the viability of C540 cells treated by Au-PTX NPs under ultrasound radiation was decreased significantly by generating more reactive oxygen species (ROS) upon STD, with representing synergism effects confirming the role of gold nanoparticles as an excellent sonosensitizer and the role of SDT as an adjunctive treatment method with chemotherapy.
纳米药物递送系统是肿瘤治疗的新策略，因为化疗药物如紫杉醇（PTX）的递送由于其较差的水溶性而存在很大的挑战。此外，声动力学疗法（SDT）是一种很有前途的方法，可以通过激活声敏剂和增强细胞膜通透性来增加所需药物的摄取、积累和分散。本文合成并表征了金-紫杉醇纳米粒子（Au-PTX NPs），以评估其对 C540 癌细胞的细胞毒性，并与游离 PTX、AuNPs 和 AuNPs+游离 PTX 在有无超声辐射的情况下进行比较。证据表明，AuNPs 的平均直径为 95.0±15.4nm，而 Au-PTX NPs 的尺寸约为 219.7±40.4nm。负的 zeta 电位结果表明纳米粒子具有高稳定性和良好的分散性。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）分析结果表明，Au-PTX NPs 比其他治疗组增加了细胞毒性，这确保了 AuNPs 作为 PTX 药物递送的有前途的纳米载体的巨大潜力。此外，在 SDT 作用下，Au-PTX NPs 处理的 C540 细胞的活性显著降低，这是由于产生了更多的活性氧（ROS），协同效应证实了金纳米粒子作为一种优异的声敏剂的作用，以及 SDT 作为化疗辅助治疗方法的作用。
