Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, Kars, Turkey.
Department of Molecular Biology and Genetic, Faculty of Science, Erzurum Technical University, Erzurum, Turkey.
Biol Trace Elem Res. 2024 Oct;202(10):4494-4507. doi: 10.1007/s12011-024-04090-y. Epub 2024 Feb 15.
Metformin is commonly prescribed to people with diabetes. Metformin has been shown in previous studies to be able to prevent the growth of cancer cells. This study aims to investigate the effects of metformin and gold nanoparticles in MCF7 breast cancer and A549 lung cell lines. The effects of metformin and gold nanoparticles on MCF7 breast cancer and A549 lung cells were determined on cells grown in 24 h cell culture. MCF-7 and A549 cells were incubated for 24 h with the treatment of escalating molar concentrations of ifosfamide. The MTT assay was used to determine the cytotoxicity of metformin toward MCF7 and A549 cell lines. The expression of Bax, BCL2, PI3K, Akt3, mTOR, Hsp60, Hsp70, and TNF-α was measured by RT-PCR. Metformin and gold nanoparticles inhibited the proliferation of MCF-7 and A549 cells in a dose and time-dependent manner with an IC50 value of 5 µM and 10 µg/mL. RT-PCR assays showed ifosfamide + metformin + gold nanoparticles significantly reduced the expression of BCL2, PI3K, Akt3, mTOR, Hsp60 and Hsp70 and increased the expression of TNF-α and Bax. The findings obtained in this study suggest that further studies should be conducted, and metformin and gold nanoparticles can be used in breast cancer and lung cancer treatments.
二甲双胍常用于治疗糖尿病患者。先前的研究表明，二甲双胍能够阻止癌细胞的生长。本研究旨在探究二甲双胍和金纳米粒子对 MCF7 乳腺癌和 A549 肺癌细胞的影响。将 MCF7 和 A549 细胞在 24 小时细胞培养中进行培养，以确定二甲双胍和金纳米粒子对 MCF7 乳腺癌和 A549 肺癌细胞的影响。用递增摩尔浓度的异环磷酰胺孵育 MCF-7 和 A549 细胞 24 小时。MTT 法测定二甲双胍对 MCF7 和 A549 细胞系的细胞毒性。通过 RT-PCR 测定 Bax、BCL2、PI3K、Akt3、mTOR、Hsp60、Hsp70 和 TNF-α 的表达。二甲双胍和金纳米粒子以剂量和时间依赖的方式抑制 MCF-7 和 A549 细胞的增殖，IC50 值分别为 5µM 和 10µg/mL。RT-PCR 检测结果表明，异环磷酰胺+二甲双胍+金纳米粒子显著降低了 BCL2、PI3K、Akt3、mTOR、Hsp60 和 Hsp70 的表达，增加了 TNF-α 和 Bax 的表达。本研究的结果表明，应进一步开展研究，二甲双胍和金纳米粒子可用于乳腺癌和肺癌的治疗。
