Xu Hongwei, Jiang Shengpan, Wang Jimin, Li Xuebing, Wu Tingwei, Xu Pengfei, Santos-Oliveira Ralph, Zhang Aohua
Medical Imaging Department, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China.
Department of Interventional Radiology, Wuhan Third Hospital, Wuhan, Hubei, 430074, China.
Anticancer Agents Med Chem. 2020;20(14):1648-1653. doi: 10.2174/1871520620666200529113818.
Lung cancer is among the most common cancers worldwide, responsible for 13% of all new cancer cases. Also, it is the leading cause of cancer death among both men and women. In this scenario, an effective and efficient treatment is required.
Production of two gold nanoparticles: 198Au and 99mTc-Au. The first one has been produced from irradiation of the 197Au in order to produce a beta-emitter gold nanoparticle for cancer therapy. The second one has been produced from the radiolabeling of gold nanoparticles with technetium 99 metastable in order to produce imaging nanoagent.
The 198Au nanoparticles were produced by irradiation and identified by hyper-purity germanium (HPGe). They were then evaluated in vitro in order to confirm the behavior on cell proliferation of lung cancer cell lines by the MTT methodology using A549 cells. The 99mTc-Au nanoparticles were produced by directradiolabeling with 99mTc and evaluated in vivo as intralesional nanoagent.
The results showed that in both cases, all the nanoparticles have performed their duties with excellence. The 198Au nanoparticles were capable to kill lung cancer cells, while 99mTc-Au was capable to image the tumor after intralesional injection. In addition, 99mTc-Au nanoparticles were useful for biodistribution assay imaging, showing the main organs responsible for the nanoparticle uptake in healthy animals.
Both gold nanoparticles showed to be a highly efficient nanoagent for both: therapy and diagnosing of lung cancer.
肺癌是全球最常见的癌症之一，占所有新癌症病例的13%。此外，它还是男性和女性癌症死亡的主要原因。在这种情况下，需要一种有效且高效的治疗方法。
制备两种金纳米颗粒：198Au和99mTc-Au。第一种是通过对197Au进行辐照制备的，以生产用于癌症治疗的β发射体金纳米颗粒。第二种是通过用99m锝对金纳米颗粒进行放射性标记制备的，以生产成像纳米剂。
198Au纳米颗粒通过辐照制备，并用高纯锗（HPGe）进行鉴定。然后在体外进行评估，以使用A549细胞通过MTT方法确认其对肺癌细胞系细胞增殖的影响。99mTc-Au纳米颗粒通过用99mTc直接放射性标记制备，并作为瘤内纳米剂进行体内评估。
结果表明，在两种情况下，所有纳米颗粒都出色地履行了其职责。198Au纳米颗粒能够杀死肺癌细胞，而99mTc-Au在瘤内注射后能够对肿瘤进行成像。此外，99mTc-Au纳米颗粒可用于生物分布测定成像，显示健康动物体内负责纳米颗粒摄取的主要器官。
两种金纳米颗粒都显示出对肺癌治疗和诊断都是高效的纳米剂。
