a Department of Thoracic Surgery, Shaanxi Provincial Tumor Hospital , Xi'an , China.
b Department of Pulmonary Disease, Jinan Municipal Hospital of Traditional Chinese Medicine , Jinan , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2171-2178. doi: 10.1080/21691401.2019.1620249.
Nanomedicine is a rapidly emerging field and is reported to be a promising tool for treating various diseases. Green synthesized nanoparticles are documented to possess a potent anticancer effect. is a Chinese plant which is also one of the components of PC-SPES and used to treat prostate cancer. In the present study, we synthesized the gold nanoparticles from (RR-AuNP) and analyzed its anticancer activity against the lung carcinoma A549 cell lines. Since lung cancer is reported to be with increased morbidity and decreased survival rate. The biosynthesized RR-AuNP were confirmed using UV-Visible spectrophotometer, size and shape of RR-AuNP were assessed by DLS, TEM and EDX. The biomolecules present in RR-AuNP and its topographical structure were detected using FTIR, SAED and AFM analysis. MTT assay was performed to detect the IC50 dose of RR-AuNP and its apoptotic effect was assessed by detecting the caspases activation, ROS generation. The anticancer effect of RR-AuNP was confirmed by DAPI staining, TUNEL assay and its molecular mechanism were confirmed by assessing the apoptotic signalling molecules protein expression. Our results illustrate that RR-AuNP showed a strong absorption peak at 550 nm and the RRAuNP were polydispersed nanospheres with size of 130 nm. RR-AuNP IC50 dose against A549 lung carcinoma cell line was detected to be at 25 µg/ml. The results of DAPI staining, TUNEL and immunoblotting analysis confirms both the 25 µg/ml and 50 µg/ml of RR-AuNP possess potent anticancer and apoptotic effect, suggesting that RR-AuNP that it may be a persuasive molecule to treat lung cancer.
纳米医学是一个快速发展的领域，被报道为治疗各种疾病的有前途的工具。绿色合成的纳米粒子被证明具有强大的抗癌作用。 是一种中国植物，也是 PC-SPES 的成分之一，用于治疗前列腺癌。在本研究中，我们从 （RR-AuNP）合成了金纳米粒子，并分析了其对肺癌 A549 细胞系的抗癌活性。由于肺癌的发病率增加和生存率降低。使用紫外可见分光光度计证实了生物合成的 RR-AuNP，使用 DLS、TEM 和 EDX 评估了 RR-AuNP 的大小和形状。使用 FTIR、SAED 和 AFM 分析检测 RR-AuNP 中的生物分子及其形貌结构。通过 MTT 测定法检测 RR-AuNP 的 IC50 剂量，并通过检测 caspase 激活、ROS 生成来评估其凋亡作用。通过 DAPI 染色、TUNEL 测定和评估凋亡信号分子蛋白表达来证实 RR-AuNP 的抗癌作用。我们的结果表明，RR-AuNP 在 550nm 处显示出强吸收峰，RR-AuNP 是具有 130nm 尺寸的多分散纳米球。RR-AuNP 对 A549 肺癌细胞系的 IC50 剂量检测为 25μg/ml。DAPI 染色、TUNEL 和免疫印迹分析的结果证实，25μg/ml 和 50μg/ml 的 RR-AuNP 均具有强大的抗癌和凋亡作用，这表明 RR-AuNP 可能是治疗肺癌的有说服力的分子。
