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一种简单而有前景的基于纸的电化学平台，用于血清学检测美洲皮肤利什曼病。

Simple and promising paper-based electrochemical platform for serological detection of American tegumentary leishmaniasis.

机构信息

Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Tucumán, Facultad de Ciencias Exactas y Tecnología, Instituto Superior de Investigaciones Biológicas, Laboratorio de Medios e Interfases, Tucumán, Argentina.

Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Salta, Facultad de Ciencias de la Salud, Instituto de Patología Experimental, Salta, Argentina.

出版信息

Mem Inst Oswaldo Cruz. 2024 Feb 12;119:e230149. doi: 10.1590/0074-02760230149. eCollection 2024.

DOI:10.1590/0074-02760230149
PMID:38359306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10868376/
Abstract

BACKGROUND

American tegumentary leishmaniasis (ATL) is an endemic neglected tropical disease (NTD), its conventional treatment is toxic, slow, and invasive. Rapid diagnosis is crucial for the clinical management of suspected patients, so the development and use of low-cost, miniaturised and portable devices could be the key.

OBJECTIVES

This work aimed to develop a simple paper-based electrochemical platform for the serological detection of ATL.

METHODS

Platform was fabricated in Whatman N°1 paper, contains a hydrophobic zone generated by wax printing, two pencil graphite electrodes, and uses specific crude extracts (CA) antigens for ATL immuno-determination. The platform performance was analysed by measuring the relative impedance change for different antigen-antibody combinations. Then, 10 serum human samples previously diagnosed by the gold standard (five positive ATL cases and five non-ATL cases) were evaluated.

FINDINGS

The platform presented a linear response for the charge transfer resistance (ΔRct) and the interface reactance (ΔXc). Also, optimal working conditions were established (1/60 serum dilution and 180 µg/mL CA concentration). Then, the platform permits to distinguish between ATL and non-ATL (p < 0.05) human serum samples.

MAIN CONCLUSIONS

Our platform could allow the diagnosis, management, and monitoring of leishmaniasis while being an extremely simple and environmentally friendly technology.

摘要

背景

美洲皮肤利什曼病（ATL）是一种地方性被忽视的热带病（NTD），其常规治疗具有毒性、缓慢且具有侵入性。快速诊断对于疑似患者的临床管理至关重要，因此开发和使用低成本、小型化和便携式设备可能是关键。

目的

本研究旨在开发一种用于 ATL 血清学检测的简单纸基电化学平台。

方法

平台由沃特曼 N°1 纸制成，包含由蜡印产生的疏水区域、两个铅笔石墨电极，并使用特定的粗提物（CA）抗原进行 ATL 免疫测定。通过测量不同抗原-抗体组合的相对阻抗变化来分析平台性能。然后，评估了 10 个人类血清样本，这些样本先前已通过金标准（5 例阳性 ATL 病例和 5 例非 ATL 病例）诊断。

发现

该平台的电荷转移电阻（ΔRct）和界面电抗（ΔXc）呈现线性响应。此外，还确定了最佳工作条件（血清稀释度为 1/60 和 CA 浓度为 180 µg/mL）。然后，该平台可区分 ATL 和非 ATL（p<0.05）人类血清样本。

主要结论

我们的平台可以在极其简单和环保的技术下允许对利什曼病进行诊断、管理和监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/cb5b83f691eb/1678-8060-mioc-119-e230149-gf7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/5ad8ba5654ff/1678-8060-mioc-119-e230149-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/ea7878cd2bef/1678-8060-mioc-119-e230149-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/494bdda96e5c/1678-8060-mioc-119-e230149-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/917021d38e9e/1678-8060-mioc-119-e230149-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/b7ea2502ab3f/1678-8060-mioc-119-e230149-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/87810233b696/1678-8060-mioc-119-e230149-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/cb5b83f691eb/1678-8060-mioc-119-e230149-gf7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/5ad8ba5654ff/1678-8060-mioc-119-e230149-gf1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/ea7878cd2bef/1678-8060-mioc-119-e230149-gf2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/494bdda96e5c/1678-8060-mioc-119-e230149-gf3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/917021d38e9e/1678-8060-mioc-119-e230149-gf4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/b7ea2502ab3f/1678-8060-mioc-119-e230149-gf5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/87810233b696/1678-8060-mioc-119-e230149-gf6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc3e/10868376/cb5b83f691eb/1678-8060-mioc-119-e230149-gf7.jpg

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