实验性免疫复合物性中耳炎：用蛋白A-金技术定位IgG
Experimental immune complex otitis media: localization of IgG by protein A-gold technique.
作者信息
Tachibana M, Morioka H, Tanimura F, Tanaka T, Machino M, Amagai T, Imanishi J, Mizukoshi O
出版信息
Auris Nasus Larynx. 1985;12 Suppl 1:S86-8. doi: 10.1016/s0385-8146(85)80110-3.
Immune complex (IC) was made of bovine serum albumin (BSA) and anti-BSA guinea pig serum, and instilled into the tubotympanic cavity of untreated guinea pigs. The location of IgG was determined by using the protein A-gold technique to trace IC in the tubotympanic mucosa of the otitis media with effusion (OME) caused by the instillation. IgG was found on the effusion, degenerated ciliated cells and on granules of goblet cells. There was no evidence of intense accumulation of IgG on the basement membrane of the mucosal epithelium or the capillaries. It is likely that IC activates complements in the tubotympanic cavity to cause OME.
免疫复合物（IC）由牛血清白蛋白（BSA）和抗BSA豚鼠血清制成，并注入未治疗的豚鼠鼓室腔。通过使用蛋白A-金技术追踪IC在因注入而导致的渗出性中耳炎（OME）鼓室黏膜中的位置来确定IgG的位置。在渗出液、变性的纤毛细胞和杯状细胞的颗粒上发现了IgG。没有证据表明IgG在黏膜上皮或毛细血管的基底膜上有强烈积聚。IC很可能在鼓室腔中激活补体从而导致OME。
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