Ryan A F, Catanzaro A, Wasserman S I, Harris J P
Ann Otol Rhinol Laryngol. 1986 May-Jun;95(3 Pt 1):242-9. doi: 10.1177/000348948609500307.
A model of secondary immune response in the middle ear (ME) was developed in the guinea pig. Animals were immunized intradermally with keyhole limpet hemocyanin (KLH) and challenged with KLH in the ME 8 weeks later. The ME displayed effusion, mucosal hyperplasia, mucosal edema, and leukocytic infiltration. These responses were maximal at 3 days postchallenge, and persisted for 2 to 3 weeks. High levels of antibody were observed in the ME effusions, and serum titers increased significantly. Immunohistochemical localization of antibody indicated that substantial free IgG was present in the submucosa, with much less in the mucosal epithelium. IgA plasma cells were observed in the submucosa at 1 week postchallenge, but no IgM was detected. In contrast to the response observed in sensitized animals, antigen presented to the ME of unimmunized control animals resulted in no effusion, minimal mucosal inflammation, and little or no antibody in either the ME or serum. However, substantial neutrophilic infiltration of the ME cavity was observed. The effusion and inflammation produced by secondary ME immune responses were not affected by tympanostomy tubes. Eustachian tube function as measured by a forced response test was not affected by ME immune response. This suggests that the immunologically induced effusion was not the result of eustachian tube obstruction. We postulate that increased vascular permeability in the ME mucosa, induced by chemical mediators, resulted in the transudation of serum.
在豚鼠身上建立了中耳（ME）二次免疫反应模型。动物经皮内注射钥孔戚血蓝蛋白（KLH）进行免疫，8周后在中耳用KLH进行激发。中耳出现积液、黏膜增生、黏膜水肿和白细胞浸润。这些反应在激发后3天达到峰值，并持续2至3周。在中耳积液中观察到高水平的抗体，血清滴度显著升高。抗体的免疫组化定位表明，黏膜下层存在大量游离IgG，而黏膜上皮中的含量则少得多。激发后1周在黏膜下层观察到IgA浆细胞，但未检测到IgM。与致敏动物中观察到的反应相反，将抗原呈递给未免疫对照动物的中耳不会导致积液、最小程度的黏膜炎症，且中耳或血清中几乎没有抗体。然而，观察到中耳腔内有大量嗜中性粒细胞浸润。中耳二次免疫反应产生的积液和炎症不受鼓膜造孔管的影响。通过强制反应试验测量的咽鼓管功能不受中耳免疫反应的影响。这表明免疫诱导的积液不是咽鼓管阻塞的结果。我们推测，化学介质诱导的中耳黏膜血管通透性增加导致了血清渗出。
