Yau Jerry Chun-Kit, Hung Ka-Lung, Ren Yikun, Kajitani Takashi, Stuart Marc C A, Leung Franco King-Chi
The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen 518057, China; State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China.
TC College Promotion Office, Open Facility Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
J Colloid Interface Sci. 2024 May 15;662:391-403. doi: 10.1016/j.jcis.2024.02.075. Epub 2024 Feb 10.
Amphiphilic molecules functionalized with photoresponsive motifs have attractive prospects for applications in smart functional bio-material ranging from cell-material interfaces to drug delivery systems owing to the precisely controllable functionality of self-assembled hierarchical supramolecular structures in aqueous media by a non-invasive light stimulation with high temporal- and spatial-resolution. However, most of reported photoresponsive amphiphiles are triggered by bio-damaging UV-light, which greatly limits the potential in bio-related applications. Herein, we present newly designed red-light controlled N,N'-diaryl-substituted indigo amphiphiles (IA), exhibiting excellent photoswitchablity and photostability with dual red-/green-light in organic media. Meanwhile, aqueous solutions of IA assembled into supramolecular structures in both microscopic and macroscopic length-scale, though the photoresponsiveness of IA is slightly compromised in aqueous media. At macroscopic length-scale, morphological changes of IA macroscopic scaffold prepared by a shear-flow method can be fine adjusted upon red-light irradiation. Moreover, the preferential attachment of live h-MSCs to IA macroscopic scaffold surface also indicates a good biocompatibility of IA macroscopic scaffold. These results provide the potential for developing the next generation of red-light controlled soft functional materials with good biocompatibility.
具有光响应基序功能化的两亲分子在智能功能生物材料领域具有诱人的应用前景，其应用范围涵盖从细胞-材料界面到药物递送系统等多个方面。这是因为在水介质中，通过具有高时空分辨率的非侵入性光刺激，自组装的分级超分子结构具有精确可控的功能。然而，大多数已报道的光响应两亲分子是由具有生物损伤性的紫外光触发的，这极大地限制了其在生物相关应用中的潜力。在此，我们展示了新设计的红光控制的N，N'-二芳基取代靛蓝两亲分子（IA），其在有机介质中对红光/绿光具有优异的光开关性和光稳定性。同时，IA的水溶液在微观和宏观长度尺度上均组装成超分子结构，尽管IA在水介质中的光响应性略有受损。在宏观长度尺度上，通过剪切流方法制备的IA宏观支架在红光照射下其形态变化可得到精细调节。此外，活的人骨髓间充质干细胞优先附着于IA宏观支架表面，这也表明IA宏观支架具有良好的生物相容性。这些结果为开发具有良好生物相容性的下一代红光控制的软功能材料提供了潜力。
