College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.
Department of Obstetrics and Gynaecology, General Hospital of Northern Theater Command, Shenyang 110016, China.
Food Chem. 2024 Jul 1;445:138651. doi: 10.1016/j.foodchem.2024.138651. Epub 2024 Feb 5.
Endogenous peptides and their parent proteins are important nutritional components with diverse biological functions. The objective of this study was to analyze and compare endogenous peptides and parent proteins found in human colostrum (HC) and human mature milk (HM) using a 4D label-free technique. In total, 5162 and 940 endogenous peptides derived from 258 parent proteins were identified in human milk by database (DB) search and de novo, respectively. Among these peptides, 2446 differentially expressed endogenous peptides with various bioactivities were identified. The Gene Ontology analysis unveiled the cellular components, biological processes, and molecular functions associated with these parent proteins. Metabolic pathway analysis suggested that neutrophil extracellular trap formation had the greatest significance with 24 parent proteins. These findings will offer a fresh perspective on the development of infant formula powder, highlighting the potential for incorporating these changes to enhance its nutritional composition and benefits.
内源性肽及其母体蛋白是具有多种生物学功能的重要营养成分。本研究旨在使用 4D 无标记技术分析和比较人初乳（HC）和人成熟乳（HM）中的内源性肽和母体蛋白。通过数据库（DB）搜索和从头测序，分别在人乳中鉴定出 5162 种和 940 种源自 258 种母体蛋白的内源性肽。在这些肽中，鉴定出了 2446 种具有各种生物活性的差异表达内源性肽。基因本体分析揭示了与这些母体蛋白相关的细胞成分、生物过程和分子功能。代谢途径分析表明，中性粒细胞胞外诱捕网的形成具有最重要的意义，涉及 24 种母体蛋白。这些发现将为婴儿配方奶粉的开发提供新的视角，强调了将这些变化纳入其中以增强其营养成分和益处的潜力。
