Panchal Priti, Rani Reeju, Kumar Rohit, Malik Seema, Mukesh Manishi, Kaushik Jai Kumar, Sodhi Monika, Mohanty Ashok Kumar, Kumar Sudarshan
ICAR-National Dairy Research Institute, Proteomics and Cell Biology Lab, Animal Biotechnology Division (ABTD) Karnal Haryana 132001 India
College of Public Health and Human Science, Oregon State University Corvalis OR 97331 USA.
RSC Adv. 2024 Sep 16;14(40):29189-29200. doi: 10.1039/d4ra03199g. eCollection 2024 Sep 12.
Bovine milk and colostrum play pivotal roles in the nutritional support of both human and bovine infants. Colostrum, the initial milk secretion, is crucial for neonatal growth, providing essential nutrients, growth factors, immunity, and defense mechanisms through a diverse array of bioactive compounds, including bioactive proteins and peptides. Peptidomics, leveraging the potential health benefits of peptides derived from food and body fluids, has become prominent in contemporary research. Endogenous peptides (EPs) have gained notable scientific and commercial interest due to their potential biofunctional significance in areas such as immune health, antimicrobial, anti-inflammatory, antihypertensive, and antioxidative studies. In this investigation, we aimed to extract and analyze low molecular weight EPs from colostrum using four distinct peptide extraction methods, previously employed for EPs extraction from other bodily fluids. The efficiency of these methods was systematically compared and analysed to identify the most effective extraction technique for maximizing the identification of low molecular weight EPs from colostrum. This study represents a pioneering effort as no prior research has systematically compared different extraction methods for low molecular weight EPs from colostrum. Given the unique physical and chemical composition of colostrum compared to milk and other body fluids, a comprehensive analysis of EPs extraction methods was deemed essential. In the present study, we successfully extracted over 3200 EPs from colostrum using trichloroacetic acid (TCA) and a molecular weight cut off (MWCO) extraction method. The findings of this study revealed the extraction of EPs from colostrum, demonstrating potential inherent bioactivities as predicted by tools.
牛乳和初乳在人类和牛犊幼崽的营养支持方面发挥着关键作用。初乳作为最初分泌的乳汁，对新生儿的生长至关重要，它通过多种生物活性化合物，包括生物活性蛋白质和肽，提供必需营养素、生长因子、免疫力和防御机制。肽组学利用源自食物和体液的肽的潜在健康益处，在当代研究中变得日益突出。内源性肽（EPs）因其在免疫健康、抗菌、抗炎、抗高血压和抗氧化研究等领域的潜在生物功能意义而引起了显著的科学和商业关注。在本研究中，我们旨在使用四种不同的肽提取方法从初乳中提取和分析低分子量内源性肽，这些方法先前用于从其他体液中提取内源性肽。我们系统地比较和分析了这些方法的效率，以确定从初乳中最大化鉴定低分子量内源性肽的最有效提取技术。本研究是一项开创性的工作，因为之前没有研究系统地比较从初乳中提取低分子量内源性肽的不同方法。鉴于初乳与牛奶和其他体液相比具有独特的物理和化学组成，对内源性肽提取方法进行全面分析被认为是必不可少的。在本研究中，我们使用三氯乙酸（TCA）和截留分子量（MWCO）提取方法成功地从初乳中提取了超过3200种内源性肽。本研究结果揭示了从初乳中提取内源性肽的情况，证明了工具预测的潜在固有生物活性。
