Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf e. V, 01328, Dresden, Germany.
Department of Visceral, Thoracic and Vascular Surgery, University Hospital and Faculty of Medicine Carl Gustav. Carus, TUD Dresden University of Technology, Germany; Else Kröner Fresenius Center for Digital Health (EKFZ), TUD Dresden University of Technology, Germany.
Biosens Bioelectron. 2024 May 1;251:116034. doi: 10.1016/j.bios.2024.116034. Epub 2024 Feb 1.
Postoperative complications after pancreatic surgery are frequent and can be life-threatening. Current clinical diagnostic strategies involve time-consuming quantification of α-amylase activity in abdominal drain fluid, which is performed on the first and third postoperative day. The lack of real-time monitoring may delay adjustment of medical treatment upon complications and worsen prognosis for patients. We report a bedside portable droplet-based millifluidic device enabling real-time sensing of drain α-amylase activity for postoperative monitoring of patients undergoing pancreatic surgery. Here, a tiny amount of drain liquid of patient samples is continuously collected and co-encapsulated with a starch reagent in nanoliter-sized droplets to track the fluorescence intensity released upon reaction with α-amylase. Comparing the α-amylase levels of 32 patients, 97 % of the results of the droplet-based millifluidic system matched the clinical data. Our method reduces the α-amylase assay duration to approximately 3 min with the limit of detection 7 nmol/s·L, enabling amylase activity monitoring at the bedside in clinical real-time. The presented droplet-based platform can be extended for analysis of different body fluids, diseases, and towards a broader range of biomarkers, including lipase, bilirubin, lactate, inflammation, or liquid biopsy markers, paving the way towards new standards in postoperative patient monitoring.
胰腺手术后的并发症很常见，有时甚至危及生命。目前的临床诊断策略包括在术后第 1 天和第 3 天对引流液中的α-淀粉酶活性进行耗时的定量分析。缺乏实时监测可能会延迟对并发症的治疗调整，从而使患者的预后恶化。我们报告了一种床边便携式液滴式毫升级微流控装置，可实时感测引流液中的α-淀粉酶活性，用于对接受胰腺手术的患者进行术后监测。在此，患者样本的少量引流液被连续收集，并与纳米级液滴中的淀粉试剂共同包封，以跟踪与α-淀粉酶反应时释放的荧光强度。通过比较 32 名患者的α-淀粉酶水平，基于液滴的毫升级微流控系统的 97%结果与临床数据相匹配。我们的方法将α-淀粉酶检测时间缩短至大约 3 分钟，检测限为 7nmol/s·L，可在临床实时床边进行淀粉酶活性监测。所提出的基于液滴的平台可扩展用于分析不同的体液、疾病，并扩展到更广泛的生物标志物分析，包括脂肪酶、胆红素、乳酸、炎症或液体活检标志物，为术后患者监测建立新标准。
