Department of Animal Biosciences, University of Guelph, Guelph, ON, N1G 2W1, USA.
CBS BioPlatforms Inc., Calgary, AL, T2C 0J7, USA.
Poult Sci. 2024 Apr;103(4):103519. doi: 10.1016/j.psj.2024.103519. Epub 2024 Feb 7.
We evaluated a single strain Bacillus subtilis BS-9 direct-fed microbial (BSDFM) isolated from camel dung in Eimeria challenged broiler chickens. Seven-hundred d-old Ross 708 male chicks were placed in pens (25 birds/pen) and allocated to 2 treatments (n = 14). From d 0 to 13, control pens received untreated water (-BSDFM), and 2 treated pens received water and 2 mL x 10 colony forming unit/bird/d (+BSDFM); daily water intake (WI) was recorded. On d 9, birds in half (+Eimeria) of pens per treatment received of 1 mL of Eimeria maxima and Eimeria acervulina oocysts orally, and the other half (-Eimeria) sterile saline solution. Birds had ad libitum access to feed and a water line from d 14. Feed intake (FI), body weight (BW) and mortality were recorded for calculating BW gain (BWG) and feed conversion ratio (FCR). On d 14 and 35, samples of birds were necropsied for organ weight and intestinal measurements. Excreta samples were collected from d 14 to 19 for oocyst count. There was no treatment effect (P > 0.05) on growth performance or WI on d 0 to 9. There were interactions between BSDFM and Eimeria on d 19 (P = 0.014) and 29 (P = 0.036) BW with unchallenged +BSDFM birds being heavier than birds in the other treatments. The main effects (P < 0.05) on d 10 to 35 FI, BW, and BWG were such that +BSDFM increased and Eimeria decreased (P < 0.01) these parameters. There was interaction (P = 0.022) between BSDFM and Eimeria on d 10 to 35 FCR such that the FCR of challenged -BSDFM birds was poor than that of unchallenged counterparts, but none differed with +BSDFM birds. There was an interaction (P = 0.039) between BSDFM and Eimeria on d 14 bursa weight with challenged birds exhibiting heavier bursa than unchallenged +BSDFM birds. Eimeria reduced (P = 0.01) and BSDFM (P = 0.002) increased the villi height to crypt depth ratio. Results showed that BSDFM supplementation via water can support the growth performance of broiler chickens challenged with Eimeria and may be a strategy to reduce adverse effects of coccidiosis.
我们评估了一株从骆驼粪便中分离出来的枯草芽孢杆菌 BS-9 单菌株，将其作为直接饲喂微生物（BSDFM），用于感染艾美耳球虫的肉鸡。700 日龄的罗斯 708 雄性小鸡被安置在鸡舍（每舍 25 只鸡）中，并分为 2 个处理组（n=14）。从第 0 天到第 13 天，对照组饮用未经处理的水（-BSDFM），2 个处理组饮用添加了 2 mL x 10 个菌落形成单位/只/天（+BSDFM）的水；每天记录饮水量（WI）。在第 9 天，每个处理组的一半（+Eimeria）鸡接受了 1 毫升的柔嫩艾美耳球虫和堆型艾美耳球虫卵囊口服接种，另一半（-Eimeria）鸡接受无菌生理盐水。从第 14 天起，鸡可自由采食饲料和饮水线。记录采食量（FI）、体重（BW）和死亡率，以计算体重增重（BWG）和饲料转化率（FCR）。在第 14 天和第 35 天，对鸡进行剖检，以测量器官重量和肠道测量。从第 14 天到第 19 天，收集粪便样本进行卵囊计数。生长性能或第 0 天到第 9 天的 WI 无处理效应（P > 0.05）。BSDFM 和艾美耳球虫之间存在交互作用（P = 0.014），第 19 天（P = 0.036）和第 29 天（P = 0.036）BW 无挑战的+BSDFM 鸡体重高于其他处理组。第 10 天到第 35 天 FI、BW 和 BWG 的主要效应（P < 0.05）是+BSDFM 增加和艾美耳球虫减少（P < 0.01）这些参数。BSDFM 和艾美耳球虫之间存在交互作用（P = 0.022），第 10 天到第 35 天 FCR 表现为，受挑战的-BSDFM 鸡的 FCR 比未受挑战的同类差，但与+BSDFM 鸡无差异。BSDFM 和艾美耳球虫之间存在交互作用（P = 0.039），第 14 天法氏囊重量表现为，受挑战的鸡法氏囊重量比未受挑战的+BSDFM 鸡重。艾美耳球虫减少（P = 0.01）和 BSDFM（P = 0.002）增加了绒毛高度与隐窝深度的比值。结果表明，BSDFM 通过饮水补充可支持感染艾美耳球虫的肉鸡的生长性能，可能是减轻球虫病不良影响的一种策略。
