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利用病毒神经示踪剂在狨猴中非侵入性的局灶性转基因传递。

Noninvasive focal transgene delivery with viral neuronal tracers in the marmoset monkey.

机构信息

Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA, USA.

Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA, USA.

出版信息

Cell Rep Methods. 2024 Feb 26;4(2):100709. doi: 10.1016/j.crmeth.2024.100709. Epub 2024 Feb 14.

DOI:10.1016/j.crmeth.2024.100709
PMID:38359822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10921014/
Abstract

We establish a reliable method for selectively delivering adeno-associated viral vectors (AAVs) across the blood-brain barrier (BBB) in the marmoset without the need for neurosurgical injection. We focally perturbed the BBB (∼1 × 2 mm) in area 8aD of the frontal cortex in four adult marmoset monkeys using low-intensity transcranial focused ultrasound aided by microbubbles. Within an hour of opening the BBB, either AAV2 or AAV9 was delivered systemically via tail-vein injection. In all four marmosets, fluorescence-encoded neurons were observed at the site of BBB perturbation, with AAV2 showing a sparse distribution of transduced neurons when compared to AAV9. The results are compared to direct intracortical injections of anterograde tracers into area 8aD and similar (albeit sparser) long-range connectivity was observed. With evidence of transduced neurons specific to the region of BBB opening as well as long-distance tracing, we establish a framework for focal noninvasive transgene delivery to the marmoset brain.

摘要

我们建立了一种可靠的方法，可在不进行神经外科注射的情况下，选择性地将腺相关病毒载体 (AAV) 递送到狨猴的血脑屏障 (BBB) 中。我们使用低强度经颅聚焦超声辅助微泡，在成年狨猴的额皮质 8aD 区局部扰乱 BBB（约 1 × 2 毫米）。在 BBB 打开后的一个小时内，通过尾静脉注射系统地递送电镜编码的 AAV2 或 AAV9。在所有四只狨猴中，在 BBB 扰乱部位都观察到了荧光编码的神经元，与 AAV9 相比，AAV2 显示出转导神经元的稀疏分布。结果与直接向 8aD 区进行顺行示踪剂的皮质内注射进行了比较，观察到类似（尽管更稀疏）的长程连接。通过证明转导神经元是特异性针对 BBB 开放区域以及长距离示踪的，我们为狨猴大脑的焦点非侵入性转基因传递建立了一个框架。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/5fee8b134bc4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/0099b39b7f0a/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/fa09ae6d660f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/179759b18b25/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/15f573a8604d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/5fee8b134bc4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/0099b39b7f0a/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/fa09ae6d660f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/179759b18b25/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/15f573a8604d/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d452/10921014/5fee8b134bc4/gr4.jpg

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本文引用的文献

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Commun Biol. 2023 Aug 2;6(1):806. doi: 10.1038/s42003-023-05185-3.
2
Local and long-distance organization of prefrontal cortex circuits in the marmoset brain.猴脑前额叶皮层回路的局部和远程组织。
Neuron. 2023 Jul 19;111(14):2258-2273.e10. doi: 10.1016/j.neuron.2023.04.028. Epub 2023 May 16.
3
BBB opening with focused ultrasound in nonhuman primates and Parkinson's disease patients: Targeted AAV vector delivery and PET imaging.
Adv Drug Deliv Rev. 2024 Aug;211:115363. doi: 10.1016/j.addr.2024.115363. Epub 2024 Jun 19.
4
Focused ultrasound-mediated delivery of viral neuronal tracers in marmosets.聚焦超声介导病毒神经元示踪剂在狨猴中的传递。
Cell Rep Methods. 2024 Feb 26;4(2):100719. doi: 10.1016/j.crmeth.2024.100719.
在非人灵长类动物和帕金森病患者中使用聚焦超声打开 BBB：靶向 AAV 载体传递和 PET 成像。
Sci Adv. 2023 Apr 21;9(16):eadf4888. doi: 10.1126/sciadv.adf4888. Epub 2023 Apr 19.
4
Efficacy of gene delivery to the brain using AAV and ultrasound depends on serotypes and brain areas.使用 AAV 和超声将基因递送到大脑的效果取决于血清型和大脑区域。
J Control Release. 2022 Nov;351:667-680. doi: 10.1016/j.jconrel.2022.09.048. Epub 2022 Oct 6.
5
Incisionless targeted adeno-associated viral vector delivery to the brain by focused ultrasound-mediated intranasal administration.经聚焦超声介导的鼻内给药实现无切口靶向腺相关病毒载体递送至脑内。
EBioMedicine. 2022 Oct;84:104277. doi: 10.1016/j.ebiom.2022.104277. Epub 2022 Sep 21.
6
Microbubble drug conjugate and focused ultrasound blood brain barrier delivery of AAV-2 SIRT-3.微泡药物偶联物和聚焦超声血脑屏障传递 AAV-2 SIRT-3。
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An open access resource for functional brain connectivity from fully awake marmosets.一个来自完全清醒的狨猴的功能性脑连接的开放获取资源。
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