基于网络药理学、分子对接和实验研究探讨总丹酚酸治疗心肌缺血再灌注损伤的作用机制。
Study on therapeutic mechanism of total salvianolic acids against myocardial ischemia-reperfusion injury based on network pharmacology, molecular docking, and experimental study.
机构信息
Institute of Basic Medical Sciences, XiYuan Hospital of China Academy of Chinese Medical Sciences, No.1 XiYuan CaoChang, Haidian District, Beijing, 100091, China; Key Laboratory of Pharmacology of Chinese Materia Medica of Beijing, No.1 XiYuan CaoChang, Haidian District, Beijing, 100091, China; Graduate School of China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Institute of Basic Medical Sciences, XiYuan Hospital of China Academy of Chinese Medical Sciences, No.1 XiYuan CaoChang, Haidian District, Beijing, 100091, China; Key Laboratory of Pharmacology of Chinese Materia Medica of Beijing, No.1 XiYuan CaoChang, Haidian District, Beijing, 100091, China.
出版信息
J Ethnopharmacol. 2024 May 23;326:117902. doi: 10.1016/j.jep.2024.117902. Epub 2024 Feb 13.
ETHNOPHARMACOLOGICAL RELEVANCE
Radix Salviae miltiorrhizae, also known as Danshen in Chinese, effectively activates the blood and resolves stasis. Total salvianolic acids (SA) is the main active ingredient of Danshen, and related preparations, such as salvianolate injection are commonly used clinically to treat myocardial ischemia-reperfusion injury (MIRI). However, the potential targets and key active ingredients of SA have not been sufficiently investigated.
AIM OF THE STUDY
This study aimed to investigate the mechanism of action of SA in treating MIRI.
MATERIALS AND METHODS
Network pharmacology and molecular docking techniques were used to predict SA targets against MIRI. The key acting pathway of SA were validated by performing experiments in a rat MIRI model.
RESULTS
Twenty potential ingredients and 54 targets of SA in treating MIRI were identified. Ingredient-target-pathway network analysis revealed that salvianolic acid B and rosmarinic acid had the highest degree value. Pathway enrichment analysis showed that SA may regulate MIRI through the IL-17 signaling pathway, and this result was confirmed in the rat MIRI experiment.
CONCLUSION
The results of this study indicate that SA may protect MIRI by regulating the IL-17 pathway.
民族药理学相关性：丹参，又称“Danshen”，有效活血化淤。丹参总酚酸（SA）是丹参的主要活性成分，相关制剂如丹参多酚酸盐注射液常用于临床治疗心肌缺血再灌注损伤（MIRI）。然而，SA 的潜在靶点和关键活性成分尚未得到充分研究。
研究目的：本研究旨在探讨 SA 治疗 MIRI 的作用机制。
材料与方法：采用网络药理学和分子对接技术预测 SA 治疗 MIRI 的靶点。通过在大鼠 MIRI 模型中进行实验，验证 SA 的关键作用途径。
结果：鉴定出 20 种 SA 治疗 MIRI 的潜在成分和 54 个靶点。成分-靶点-通路网络分析显示，丹酚酸 B 和迷迭香酸的度数值最高。通路富集分析表明，SA 可能通过 IL-17 信号通路调节 MIRI，这一结果在大鼠 MIRI 实验中得到了验证。
结论：本研究结果表明，SA 可能通过调节 IL-17 通路来保护 MIRI。
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