检测菲律宾宿雾市选定地点白纹伊蚊中基孔肯雅热、登革热和寨卡病毒受湿度、降雨量、温度和季节的影响。

Detecting the impacts of humidity, rainfall, temperature, and season on chikungunya, dengue and Zika viruses in Aedes albopictus mosquitoes from selected sites in Cebu city, Philippines.

机构信息

Mosquito Research Laboratory, Department of Biology, University of San Carlos- Talamban Campus, 6000, Cebu city, Philippines.

Department of Science and Technology, Science Education Institute, Taguig City, Metro Manila 1631, Philippines.

出版信息

Virol J. 2024 Feb 15;21(1):42. doi: 10.1186/s12985-024-02310-4.

DOI:10.1186/s12985-024-02310-4

PMID:38360693

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10870450/

Abstract

BACKGROUND

Aedes albopictus is the secondary vector for dengue virus (DENV) in the Philippines, and also harbors chikungunya (CHIKV) and Zika (ZIKV) viruses. This study aimed to determine the minimum infection rates (MIRs) of CHIKV, DENV serotypes, and ZIKV in Ae. albopictus collected from selected two-site categories by altitude (highland [H] and lowland [L] sites) in Cebu city, Philippines during the wet (WS) and dry seasons (DS) of 2021-2022, and to explore the relationships between these arboviral MIRs and the local weather.

METHODS

The viral RNA extracts in pooled and reared adult Ae. albopictus collected during the DS and WS from two-site categories were subjected to RT-PCR to amplify and detect gene loci specific for CHIKV, DENV-1 to DENV-4, and ZIKV and analyzed with the weather data.

RESULTS

The range of CHIKV MIRs was higher in the WS (13.61-107.38 infected individuals per 1,000 mosquitoes) than in the DS (13.22-44.12), but was similar between the two-site categories. Rainfall (RF) influenced the CHIKV MIR. The MIR ranges of both DENV-2 (WS: H = 0, L = 0; DS: H = 0-5.92; L = 0-2.6) and DENV-4 (WS: H = 0, L = 0-2.90; DS: H = 2.96-6.13, L = 0-15.63) differed by season but not between the two-site categories. Relative humidity (RH), RF, and temperature did not influence DENVs' MIRs. The MIR range of ZIKV was similar in both seasons (WS: 11.36-40.27; DS: 0-46.15) and two-site categories (H = 0-90.91, L = 0-55.56). RH and temperature influenced ZIKV MIR.

CONCLUSIONS

RF influenced CHIKV MIR in Ae. albopictus, whereas RH and temperature influenced that of ZIKV. Season influenced the MIRs of CHIKV and DENVs but not in ZIKV. Ae. albopictus were co-infected with CHIKV, DENVs, and ZIKV in both highland and lowland sites in Cebu city. Recommendations include all-year-round implementation of the Philippine Department of Health's 4S enhanced strategy and installation of water pipelines in rural highlands for vector and disease control. Our findings are relevant to protect public health in the tropics in this climate change.

摘要

背景

白纹伊蚊是菲律宾登革热病毒（DENV）的次要传播媒介，也是基孔肯雅热（CHIKV）和寨卡（ZIKV）病毒的宿主。本研究旨在确定 2021-2022 年菲律宾宿雾市两个海拔高度（高地 [H] 和低地 [L] 地点）的两个地点类别（高山 [H] 和低地 [L] 地点）收集的白纹伊蚊中的 CHIKV、DENV 血清型和 ZIKV 的最小感染率（MIR），并探讨这些虫媒病毒 MIR 与当地天气之间的关系。

方法

在 DS 和 WS 期间，从两个地点类别中收集的经饲养的成年白纹伊蚊的病毒 RNA 提取物通过 RT-PCR 进行扩增和检测 CHIKV、DENV-1 至 DENV-4 和 ZIKV 的基因座特异性，并与天气数据进行分析。

结果

WS（每 1000 只蚊子中有 13.61-107.38 只感染个体）中的 CHIKV MIR 范围高于 DS（13.22-44.12），但两个地点类别之间相似。降雨量（RF）影响 CHIKV MIR。DENV-2（WS：H=0，L=0；DS：H=0-5.92；L=0-2.6）和 DENV-4（WS：H=0，L=0-2.90；DS：H=2.96-6.13，L=0-15.63）的 MIR 范围因季节而异，但不因地点类别而异。相对湿度（RH）、RF 和温度均不影响 DENVs 的 MIR。ZIKV 的 MIR 范围在两个季节（WS：11.36-40.27；DS：0-46.15）和两个地点类别（H=0-90.91，L=0-55.56）均相似。RH 和温度影响 ZIKV MIR。

结论

RF 影响白纹伊蚊中的 CHIKV MIR，而 RH 和温度影响 ZIKV 的 MIR。季节影响 CHIKV 和 DENVs 的 MIR，但不影响 ZIKV。在宿雾市的高地和低地地点，白纹伊蚊同时感染 CHIKV、DENV 和 ZIKV。建议包括全年实施菲律宾卫生部的 4S 强化策略和在农村高地安装水管以控制病媒和疾病。我们的研究结果与保护热带地区的公共卫生有关在这种气候变化下。