Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.
Vet Res. 2024 Feb 15;55(1):19. doi: 10.1186/s13567-024-01273-2.
A positive Mycoplasma hyopneumoniae PCR result in a clinical specimen may eventually represent the mere detection of non-viable bacteria, complicating the diagnostic interpretation. Thus, the objective of this study was to evaluate the PCR detection of non-viable M. hyopneumoniae and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivated M. hyopneumoniae and were sampled for up to 14 days. Mycoplasma hyopneumoniae was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viable M. hyopneumoniae DNA was rapidly sensed and cleared.
临床样本中肺炎支原体 PCR 阳性结果最终可能仅代表检测到非存活细菌，从而使诊断解释变得复杂。因此，本研究的目的是评估活猪中不可培养肺炎支原体及其游离细胞外 DNA 的 PCR 检测。将活的或灭活的肺炎支原体接种给猪，并在最多 14 天内进行采样。在接种灭活细菌的猪中，任何时间点都未通过 PCR 检测到肺炎支原体，表明在健康猪中，非存活的肺炎支原体 DNA 很快被感知并清除。
