Mishra Ayushi, Kumar Sunil, Singh Anchal
Department of Biochemistry, Institute of Science, Banaras Hindu University Varanasi 221005 U.P. India
RSC Adv. 2024 Feb 14;14(9):5893-5906. doi: 10.1039/d3ra08702f.
The available anti-filarial medications are largely ineffective against adult filarial worms. Also, these drugs have several drawbacks such as toxicity and development of resistance owing to long-term usage. Green nanomedicine may offer better solutions for Lymphatic Filariasis treatment due to its tiny size, biocompatibility, and better penetration at considerably lower costs with higher therapeutic efficacy. In the present study, silver nanoparticles (OSAgNPs) were bio-synthesized and their anti-filarial efficacy was evaluated against adult filarial parasites. The green nanoparticles were characterized by UV-VIS spectroscopy, XRD, FTIR, SEM, and TEM analysis. The OSAgNPs significantly affected the motility and viability of adult parasites after 4 h of incubation at concentrations higher than 0.5 μg ml. Proteomics analysis by high resolution accurate mass spectrometry revealed that 213 proteins were differentially expressed following OSAgNP treatment. Mostly these DEPs belonged to the many biochemical and molecular pathways of parasites such as muscle proteins, antioxidant proteins, heat shock proteins, signal recognition proteins, and energy metabolism-related proteins. Undoubtedly, this study will open new avenues for the development of novel anti-filarial drugs based on green nanoparticles.
现有的抗丝虫药物对成年丝虫大多无效。此外，这些药物存在一些缺点，如毒性以及长期使用会产生耐药性。绿色纳米医学因其微小的尺寸、生物相容性、能以相当低的成本实现更好的渗透且具有更高的治疗效果，可能为淋巴丝虫病的治疗提供更好的解决方案。在本研究中，生物合成了银纳米颗粒（OSAgNPs），并评估了其对成年丝虫寄生虫的抗丝虫功效。通过紫外可见光谱、X射线衍射、傅里叶变换红外光谱、扫描电子显微镜和透射电子显微镜分析对绿色纳米颗粒进行了表征。在浓度高于0.5μg/ml的情况下孵育4小时后，OSAgNPs对成年寄生虫的运动性和活力产生了显著影响。通过高分辨率精确质谱进行的蛋白质组学分析表明，经OSAgNP处理后有213种蛋白质差异表达。这些差异表达蛋白大多属于寄生虫的许多生化和分子途径，如肌肉蛋白、抗氧化蛋白、热休克蛋白、信号识别蛋白和能量代谢相关蛋白。毫无疑问，这项研究将为基于绿色纳米颗粒的新型抗丝虫药物的开发开辟新途径。
